Erratum: distinct loops in arrestin differentially regulate ligand binding within the gpcr opsin

_Nature Communication_ 3: Article number: 995 (2012); Published: 7 Aug 2012; Updated: 11 Dec 2012. In this Article, there are several instances where all-_trans_-retinal is incorrectly

referred to as all-_trans_-retinol and all-_trans_-retinol is incorrectly abbreviated to ATR. The following sentences, and the revised figure below, are correct. In the abstract: Here we

show that rod arrestin induces uptake of the agonist all-_trans_-retinal in only half the population of phosphorylated opsin in the native membrane. In the abstract: Such a mechanism would

protect the rod cell in bright light by concurrently sequestering toxic all-_trans_-retinal and allowing regeneration with 11-_cis_-retinal. In the Results: Incidentally, both

all-_trans_-retinol and beta-ionone also induced arrestin binding to OpsP with an apparent Kd ∼40 times higher than that for ATR (Fig. 3e). On the _x_ axis of Fig. 3a: All-_trans_-retinal. 

In the legend to Fig. 3e: All-_trans_-retinol and beta-ionone were titrated against 4 μM arrestin+4 μM OpsP (100% phosphorylated) in isotonic buffer, and arrestin binding was measured by

pull down. In the legend to Fig. 4: All-_trans_-retinol formation after addition of NADPH to samples of OpsP (2 μM) and ATR (4 μM) was monitored in the absence (dark red) or presence of 2 μM

unlabelled arrestin (red). Experiments were performed in 50 mM HEPES buffer pH 7 without salt, 35 °C. In the legend to Table 1: ATR, all-_trans_-retinal; NA, not applicable; OpsP,

phosphorylated opsin. The correct version of Fig. 3 and its legend follows. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Institut für Medizinische Physik und Biophysik (CC2), Charité -

Universitätsmedizin Berlin, Charitéplatz 1, D-10117 Berlin, Germany., Martha E. Sommer, Klaus Peter Hofmann & Martin Heck * Zentrum für Biophysik und Bioinformatik, Humboldt-Universität

zu Berlin, Invalidenstrasse 42, D-10115 Berlin, Germany., Klaus Peter Hofmann Authors * Martha E. Sommer View author publications You can also search for this author inPubMed Google Scholar

* Klaus Peter Hofmann View author publications You can also search for this author inPubMed Google Scholar * Martin Heck View author publications You can also search for this author inPubMed

 Google Scholar CORRESPONDING AUTHOR Correspondence to Martha E. Sommer. ADDITIONAL INFORMATION The online version of the original article can be found at 10.1038/ncomms2000 RIGHTS AND

PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Sommer, M., Hofmann, K. & Heck, M. Erratum: Distinct loops in arrestin differentially regulate ligand binding

within the GPCR opsin. _Nat Commun_ 3, 1273 (2012). https://doi.org/10.1038/ncomms2284 Download citation * Published: 11 December 2012 * DOI: https://doi.org/10.1038/ncomms2284 SHARE THIS

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