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The goal of high-throughput screening (HTS) from the perspective of the biologist is to identify a highly specific small molecule that can be used to inhibit a protein in its normal
biological context. Although several useful small molecules have been identified with HTS, there are many challenges to be considered when contemplating a screen, especially by those
unfamiliar with chemical biology.
The authors thank D. Halpin and J. Williams for useful discussions.
Department of Molecular and Cell Biology, University of California, Berkeley, 94720, California, USA
Department of Pharmaceutical Chemistry, University of California, San Francisco, 94143, California, USA
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