Mussel protein adhesion depends on interprotein thiol-mediated redox modulation

ABSTRACT Mussel adhesion is mediated by foot proteins (mfps) rich in a catecholic amino acid, 3,4-dihydroxyphenylalanine (dopa), capable of forming strong bidentate interactions with a

variety of surfaces. A tendency toward facile auto-oxidation, however, often renders dopa unreliable for adhesion. We demonstrate that mussels limit dopa oxidation during adhesive plaque

formation by imposing an acidic, reducing regime based on the thiol-rich mfp-6, which restores dopa by coupling the oxidation of thiols to dopaquinone reduction. Access through your

institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print

issues and online access $259.00 per year only $21.58 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to

local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT

BEING VIEWED BY OTHERS MOLECULAR DESIGN PRINCIPLES OF LYSINE-DOPA WET ADHESION Article Open access 04 August 2020 CATECHOL REDOX MAINTENANCE IN MUSSEL ADHESION Article 15 January 2025

SOLUTION STRUCTURE OF RECOMBINANT PVFP-5Β REVEALS INSIGHTS INTO MUSSEL ADHESION Article Open access 25 July 2022 REFERENCES * Zhao, H. & Waite, J.H. _J. Biol. Chem._ 281, 26150–26158

(2006). Article  CAS  PubMed  Google Scholar  * Zhao, H., Robertson, N.B., Jewhurst, S. & Waite, J.H. _J. Biol. Chem._ 281, 11090–11096 (2006). Article  CAS  PubMed  Google Scholar  *

Lee, H., Scherer, N.F. & Messersmith, P.B. _Proc. Natl. Acad. Sci. USA_ 103, 12999–13003 (2006). Article  CAS  PubMed  Google Scholar  * Stewart, R.J. _Appl. Microbiol. Biotechnol._ 89,

27–33 (2011). Article  CAS  PubMed  Google Scholar  * Proudfoot, G.M. & Ritchie, I.M. _Aust. J. Chem._ 36, 885–894 (1983). Article  CAS  Google Scholar  * Yu, J., Wei, W., Danner, E.,

Israelachvili, J.N. & Waite, J.H. _Adv. Mat_. 23, 2362–2366 (2011). Article  CAS  Google Scholar  * Rzepecki, L.M., Nagafuchi, T. & Waite, J.H. _Arch. Biochem. Biophys._ 285, 17–26

(1991). Article  CAS  PubMed  Google Scholar  * Liu, B., Burdine, L. & Kodadek, T. _J. Am. Chem. Soc._ 129, 12348–12349 (2007). Article  CAS  PubMed  PubMed Central  Google Scholar  *

Silverman, H.G. & Roberto, F.F. _Mar. Biotechnol._ 9, 661–681 (2007). Article  CAS  PubMed  PubMed Central  Google Scholar  * Hwang, D.S. et al. _J. Biol. Chem._ 285, 25850–25858 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar  * Lin, Q. et al. _Proc. Natl. Acad. Sci. USA_ 104, 3782–3786 (2007). Article  CAS  PubMed  Google Scholar  * Waite, J.H. _Int. J.

Adhes. Adhes._ 7, 9–14 (1987). Article  CAS  Google Scholar  * Tamarin, A., Lewis, P. & Askey, J. _J. Morphol._ 149, 199–221 (1976). Article  CAS  PubMed  Google Scholar  * Sagert, J.

& Waite, J.H. _J. Exp. Biol._ 212, 2224–2236 (2009). Article  CAS  PubMed  PubMed Central  Google Scholar  * Freund, J. & Kalbitzer, H.R. _J. Biomol. NMR_ 5, 321–322 (1995). Article

  CAS  PubMed  Google Scholar  * Hunsaker, D.B. & Schenk, G.H. _Talanta_ 30, 475–480 (1983). Article  CAS  PubMed  Google Scholar  * Jensen, K.S., Hansen, R.E. & Winther, J.R.

_Antioxid. Redox Signal._ 11, 1047–1058 (2009). Article  CAS  PubMed  Google Scholar  * Brandes, N., Schmitt, S. & Jacob, U. _Antioxid. Redox Signal._ 11, 997–1014 (2009). Article  CAS 

PubMed  PubMed Central  Google Scholar  * Anderson, T.H. et al. _Adv. Funct. Mater._ 20, 4196–4205 (2010). Article  CAS  PubMed  PubMed Central  Google Scholar  * Israelachvili, J.N.

_Intermolecular and Surface Forces_ 3rd edn. (Elsevier, London, 2010). * Inaba, K. _Genes Cells_ 15, 935–943 (2010). Article  CAS  PubMed  Google Scholar  * McDowell, L.M., Burzio, L.A.,

Waite, J.H. & Schaefer, J. _J. Biol. Chem._ 274, 20293–20295 (1999). Article  CAS  PubMed  Google Scholar  * Zeng, H., Hwang, D.S., Israelachvili, J.N. & Waite, J.H. _Proc. Natl.

Acad. Sci. USA_ 107, 12850–12853 (2010). Article  CAS  PubMed  Google Scholar  * Ohkawa, K., Nagai, T., Nishida, A. & Yamamoto, H. _J. Adhes._ 85, 770–791 (2009). Article  CAS  Google

Scholar  Download references ACKNOWLEDGEMENTS We thank the US National Institutes of Health (R01 DE018468), the National Science Foundation Materials Science & Engineering Research

Center Program (DM R05-20415) and the Human Frontiers of Science Program for supporting this research. C. Thorpe provided helpful insights about thiol chemistry. S. Nicklisch and P. Schmitt

introduced us to the DPPH assay. AUTHOR INFORMATION Author notes * Jing Yu and Wei Wei: These authors contributed equally to this work. AUTHORS AND AFFILIATIONS * Department of Chemical

Engineering, University of California, Santa Barbara, Santa Barbara, California, USA., Jing Yu & Jacob N Israelachvili * Materials Research Laboratory, University of California, Santa

Barbara, Santa Barbara, California, USA., Wei Wei * Department of Molecular, Cell & Developmental Biology, University of California, Santa Barbara, Santa Barbara, California, USA., Eric

Danner, Rebekah K Ashley & J Herbert Waite Authors * Jing Yu View author publications You can also search for this author inPubMed Google Scholar * Wei Wei View author publications You

can also search for this author inPubMed Google Scholar * Eric Danner View author publications You can also search for this author inPubMed Google Scholar * Rebekah K Ashley View author

publications You can also search for this author inPubMed Google Scholar * Jacob N Israelachvili View author publications You can also search for this author inPubMed Google Scholar * J

Herbert Waite View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS J.Y. designed and performed the SFA experiments; W.W. purified and modified

proteins; E.D. and R.K.A. contributed protein; J.H.W. wrote the manuscript and designed and co-supervised the whole project with J.N.I., who helped analyze the results. CORRESPONDING AUTHORS

Correspondence to Jacob N Israelachvili or J Herbert Waite. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. SUPPLEMENTARY INFORMATION

SUPPLEMENTARY TEXT AND FIGURES Supplementary Results and Supplementary Methods (PDF 608 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Yu, J., Wei,

W., Danner, E. _et al._ Mussel protein adhesion depends on interprotein thiol-mediated redox modulation. _Nat Chem Biol_ 7, 588–590 (2011). https://doi.org/10.1038/nchembio.630 Download

citation * Received: 09 March 2011 * Accepted: 21 May 2011 * Published: 31 July 2011 * Issue Date: September 2011 * DOI: https://doi.org/10.1038/nchembio.630 SHARE THIS ARTICLE Anyone you

share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the

Springer Nature SharedIt content-sharing initiative