Regulation of gene expression in vivo following transduction by two separate raav vectors

ABSTRACT Control of gene expression is important to gene therapy for purposes of both dosing and safety. In vivo regulation of gene expression was demonstrated following co-injection of two

separate recombinant adeno-associated virus vectors, one encoding an inducible murine erythropoietin transgene and the other a transcriptional activator, directly into the skeletal muscle of

adult immunocompetent mice. Transcription was controlled by systemic administration or withdrawal of tetracycline over an 18 week period, demonstrating that the two vectors were capable of

transducing the same cell. Cellular or humoral immune responses against the transactivator protein were not detected. Access through your institution Buy or subscribe This is a preview of

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AAV TRANSDUCTION IN MICE THROUGH ENGINEERED RECEPTOR EXPRESSION Article Open access 08 June 2023 LARGE-SCALE DISCOVERY OF POTENT, COMPACT AND ERYTHROID SPECIFIC ENHANCERS FOR GENE THERAPY

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Scholar  Download references AUTHOR INFORMATION Author notes * Richard O. Snyder: (e-mail: [email protected]). AUTHORS AND AFFILIATIONS * Cell Genesys Inc., Foster City, CA, 94404

Katherine G. Rendahl, Stuart E. Leff, Gillis R. Otten, Melinda Van Roey, Brian A. Donahue, Ronald J. Mandel & Richard O. Snyder * Institut Pasteur, Laboratoire RTC, Dept.

SIDA/Retrovirus, 75015, Paris, France S. Kaye Spratt, Delphine Bohl, Lawrence K. Cohen & Olivier Danos Authors * Katherine G. Rendahl View author publications You can also search for

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ARTICLE CITE THIS ARTICLE Rendahl, K., Leff, S., Otten, G. _et al._ Regulation of gene expression in vivo following transduction by two separate rAAV vectors. _Nat Biotechnol_ 16, 757–761

(1998). https://doi.org/10.1038/nbt0898-757 Download citation * Received: 14 April 1998 * Accepted: 22 June 1998 * Issue Date: 01 August 1998 * DOI: https://doi.org/10.1038/nbt0898-757 SHARE

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