Retroviral vector for quiescent cells?


Play all audios:

Access through your institution Buy or subscribe Most retroviral vectors used for gene therapy are derived from C-type viruses, which cannot infect quiescent cells. Lentiviruses such as


HIV-1, on the other hand, can infect quiescent cells, but concerns have been raised about their safety for human gene therapy. In this issue, Parveen et al. (see p. 623) describe how they


developed an engineered retroviral vector that has the safety features of spleen necrosis virus (SNV), a C-type retroviral vector, and that can efficiently infect quiescent cells. Previous


studies have suggested that HIV-1 can infect nondividing cells with the help of a nuclear localization sequence (NLS) located in the matrix protein. The matrix proteins of SNV and HIV-1 are


structurally similar, so the researchers tried mutating residues in the region of the SNV matrix protein analogous to the NLS in HIV-1. They found that by altering just two amino acids, they


could produce a C-type retroviral vector capable of infecting growth-arrested human T lymphocytes and quiescent primary monocyte-derived macrophages. In addition to its implications for


gene therapy, the results support the notion that the NLS is sufficient to mediate the ability of HIV-1 to infect nondividing cells. This is a preview of subscription content, access via


your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print issues and online access $209.00 per year only $17.42 per issue Learn more Buy this


article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in


* Learn about institutional subscriptions * Read our FAQs * Contact customer support Authors * Meeghan Sinclair View author publications You can also search for this author inPubMed Google


Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Sinclair, M. Retroviral vector for quiescent cells?. _Nat Biotechnol_ 18, 587 (2000).


https://doi.org/10.1038/76399 Download citation * Issue Date: June 2000 * DOI: https://doi.org/10.1038/76399 SHARE THIS ARTICLE Anyone you share the following link with will be able to read


this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative