Crystal structure of a catalytic intermediate of the maltose transporter

ABSTRACT The maltose uptake system of _Escherichia coli_ is a well-characterized member of the ATP-binding cassette transporter superfamily. Here we present the 2.8-Å crystal structure of

the intact maltose transporter in complex with the maltose-binding protein, maltose and ATP. This structure, stabilized by a mutation that prevents ATP hydrolysis, captures the ATP-binding

cassette dimer in a closed, ATP-bound conformation. Maltose is occluded within a solvent-filled cavity at the interface of the two transmembrane subunits, about halfway into the lipid

bilayer. The binding protein docks onto the entrance of the cavity in an open conformation and serves as a cap to ensure unidirectional translocation of the sugar molecule. These results

provide direct evidence for a concerted mechanism of transport in which solute is transferred from the binding protein to the transmembrane subunits when the cassette dimer closes to

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Scholar  Download references ACKNOWLEDGEMENTS We thank the beamline staff at the Advanced Photon Source beamline 23-ID for assistance with data collection, and R. MacKinnon, H. Shuman, D.

Yernool and C. Orelle for discussions. This work was supported by NIH grants (J.C., A.L.D. and F.A.Q.), the Welch Foundation (F.A.Q.), the Pew Scholar Program (J.C.) and a postdoctoral

fellowship from American Heart Association (M.L.O). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biological Sciences,, Michael L. Oldham, Dheeraj Khare & Jue Chen *

Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA, Amy L. Davidson * Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of

Medicine, Houston, Texas 77030, USA, Florante A. Quiocho Authors * Michael L. Oldham View author publications You can also search for this author inPubMed Google Scholar * Dheeraj Khare View

author publications You can also search for this author inPubMed Google Scholar * Florante A. Quiocho View author publications You can also search for this author inPubMed Google Scholar *

Amy L. Davidson View author publications You can also search for this author inPubMed Google Scholar * Jue Chen View author publications You can also search for this author inPubMed Google

Scholar CORRESPONDING AUTHOR Correspondence to Jue Chen. SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION The file contains Supplementary Figures 1-5 and Legends, Supplementary Tables 1-2

and additional references. (PDF 762 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Oldham, M., Khare, D., Quiocho, F. _et al._ Crystal structure of

a catalytic intermediate of the maltose transporter. _Nature_ 450, 515–521 (2007). https://doi.org/10.1038/nature06264 Download citation * Received: 13 August 2007 * Accepted: 17 September

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