Multilineage differentiation of dental pulp stem cells from green fluorescent protein transgenic mice


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ABSTRACT AIM The aim of this study was to confirm the multilineage differentiation ability of dental pulp stem cells (DPSCs) from green fluorescent protein (GFP) transgenic mice. The


expression of GFP in DPSCs was also observed during differentiation. METHODOLOGY DPSCs were harvested from the dental pulp tissue of transgenic nude mice, and then transferred to osteogenic,


adipogenic, and chondrogenic media. The morphological characterization of induced cells was observed by microscopy and histological staining. The expression of marker genes was measured by


RT‐PCR. RESULTS The endogenous GFP and multilineage potential of transgenic DPSCs had no influence on each other. Moreover, the results of fluorescence microscopic imaging suggest that there


was no significant decline of GFP expression during DPSCs differentiation. CONCLUSION As the population of GFP labeled DPSCs can be easily identified, this will be a promising method for


tracking DPSCs _in vivo_. You have full access to this article via your institution. Download PDF SIMILAR CONTENT BEING VIEWED BY OTHERS DIFFERENTIATION OF HUMAN ADULT-DERIVED STEM CELLS


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AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Orthopaedic Surgery, Mass General Hospital for Children and the Pediatric Orthopaedic Laboratory for Tissue Engineering, Harvard


Medical School, Boston, Massachusetts, USA Brian E Grottkau, P Prasad Purudappa & Yun‐feng Lin Authors * Brian E Grottkau View author publications You can also search for this author


inPubMed Google Scholar * P Prasad Purudappa View author publications You can also search for this author inPubMed Google Scholar * Yun‐feng Lin View author publications You can also search


for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Yun‐feng Lin. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Grottkau,


B., Purudappa, P. & Lin, Y. Multilineage Differentiation of Dental Pulp Stem Cells from Green Fluorescent Protein Transgenic Mice. _Int J Oral Sci_ 2, 21–27 (2010).


https://doi.org/10.4248/IJOS10015 Download citation * Received: 12 January 2010 * Revised: 09 February 2010 * Accepted: 09 February 2010 * Published: 01 March 2010 * Issue Date: 01 March


2010 * DOI: https://doi.org/10.4248/IJOS10015 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not


currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * dental pulp stem cells * multilineage differentiation


* green fluorescent protein