Association of sequence variations in the gene encoding insulin-like growth factor binding protein 5 with adiponectin

ABSTRACT BACKGROUND: Insulin-like growth factor binding protein 5 (IGFBP5) binds to IGF and thus modulates IGF signaling pathway. We have shown earlier that the _IGFBP5_ gene was

downregulated in the adipose tissue after 12-week carbohydrate diet with low insulinemic response. OBJECTIVE: The aim was to examine the putative contribution of genetic variation of the

_IGFBP5_ gene to the characteristics of metabolic syndrome and incidence of type 2 diabetes (T2DM) in the Finnish Diabetes Prevention Study (DPS). METHODS: DPS is a longitudinal study where

522 subjects with impaired glucose tolerance were randomized to either lifestyle intervention group or control group. DNA was available from 507 subjects (mean body mass index (BMI) 31.2±4.5

 kg/m2, age 55±7 years). The eight single-nucleotide polymorphisms (SNPs) were selected from HapMap database and genotyped by Taqman allelic discrimination protocol. The main results were

confirmed in a larger cross-sectional study population (METSIM). In addition, the gene expression of _IGFBP5_ was studied in two previously published study populations (FUNGENUT and GENOBIN)

of 124 subjects with insulin resistance (BMI 32.2±3.5 kg/m2, age 57.7±7.4 years). RESULTS: Three out of eight _IGFBP5_ markers (rs9341234, rs3276 and rs11575134) were significantly

associated with circulating adiponectin concentrations in men. Furthermore, mRNA expression studies of subcutaneous adipose tissue showed that mRNA concentrations of _IGFBP5_ correlated with

adiponectin concentrations in all subjects and in women. None of the _IGFBP5_ SNPs were associated with T2DM. CONCLUSIONS: Our findings show that _IGFBP5_ has a gender-specific association

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School of Public Health and Clinical Nutrition, University of Kuopio, Kuopio, Finland P Kallio, A-M Tolppanen, M Kolehmainen, K Poutanen, L Pulkkinen & M Uusitupa * VTT, Espoo, Finland K

Poutanen * Department of Epidemiology and Health Promotion, Diabetes and Genetic Epidemiology Unit, National Public Health Institute, Helsinki, Finland J Lindström & J Tuomilehto *

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CORRESPONDING AUTHOR Correspondence to P Kallio. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Kallio, P., Tolppanen, AM., Kolehmainen, M. _et al._

Association of sequence variations in the gene encoding insulin-like growth factor binding protein 5 with adiponectin. _Int J Obes_ 33, 80–88 (2009). https://doi.org/10.1038/ijo.2008.196

Download citation * Received: 30 May 2008 * Revised: 09 September 2008 * Accepted: 21 September 2008 * Published: 28 October 2008 * Issue Date: January 2009 * DOI:

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currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * genotyping * _IGFBP5_ * adiponectin * association *

metabolic syndrome