Detection of genomic regions differentiating two closely related oak species quercus petraea (matt. ) liebl. And quercus robur l.

ABSTRACT Genomic regions differentiating _Quercus petraea_ and _Quercus robur_ were detected by screening 2800 PCR amplification products using random primers on 22 trees of each species

sampled in 11 natural populations. Only two per cent of the amplified fragments exhibited significant frequency differences between the two species and none of them was specific to a

species. The nucleotide divergence between the two species estimated with RAPD data was 0.5 per cent in the overall genome and increased to 3.3 per cent in the discriminant regions.

Twenty-three informative fragments were cloned and partially sequenced. New primers were derived from these sequences to obtain Sequence Characterized Amplified Region (SCAR) fragments.

Southern blot experiments indicated that the SCARs were generally in low copy number in the genome. A search for similarity between SCAR sequences and sequences contained in data banks

revealed that three of them corresponded to known DNA sequences. SIMILAR CONTENT BEING VIEWED BY OTHERS A SET OF SSR MARKERS TO CHARACTERIZE GENETIC DIVERSITY IN ALL _VIBURNUM_ SPECIES

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AUTHORS AND AFFILIATIONS * I.N.R.A., Laboratoire de génétique et amélioration des arbres forestiers, B.P.45, Gazinet Cedex, F-33611, France C Bodénès, S Joandet & A Kremer * I.N.R.A.,

Laboratoire de biologie cellulaire et moléculaire, B.P. 81, Villenave D'Ornon Cedex, F-33883, France F Laigret Authors * C Bodénès View author publications You can also search for this

author inPubMed Google Scholar * S Joandet View author publications You can also search for this author inPubMed Google Scholar * F Laigret View author publications You can also search for

this author inPubMed Google Scholar * A Kremer View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to A Kremer. RIGHTS

AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Bodénès, C., Joandet, S., Laigret, F. _et al._ Detection of genomic regions differentiating two closely related

oak species _Quercus petraea_ (Matt.) Liebl. and _Quercus robur_ L.. _Heredity_ 78, 433–444 (1997). https://doi.org/10.1038/hdy.1997.67 Download citation * Received: 28 May 1996 * Issue

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nucleotide divergence * oaks * RAPD * SCAR