Inheritance of the amplified esterase genes responsible for insecticide resistance in myzus persicae (homoptera: aphididae)

ABSTRACT Insecticide-resistant and susceptible clones of _Myzus persicae_ were induced to produce sexual morphs and crossed in the laboratory. Progeny clones were analysed for karyotype,

esterase (E4, FE4 or S) gene type and activity, and amplified _E4_ and _FE4_ genes were located on their chromosomes by fluorescence _in situ_ hybridization (FISH). Amplified _FE4_ genes of

resistant parent clones (800F and French R) were inherited according to expectations. Chromosomal locations of these genes (on autosomes 1 and 3 in 800F, and on 1 and 2 in French R) were

confirmed by FISH analysis of progeny that had inherited an autosome 2 marker (a dissociation) from the susceptible parent (DS). Inheritance of amplified _E4_ genes could not be studied

directly as none of the available clones was able to produce mating females. Males from two clones with amplified _E4_ genes (and with the A1,3 translocation that is common to all

E4-producing genotypes) were therefore mated with females from clones with amplified _FE4_ genes at known chromosomal locations. Progeny were obtained with both _E4_ and _FE4_ genes, a

combination not yet found in nature. Analysis of F1 and subsequent generations confirmed that the amplified _E4_ site on autosome 3T is close to the translocation breakpoint, and apparently

coallelic with the amplified _FE4_ site on the normal autosome 3 inherited from 800F. One of the translocated parent clones (4156) had two additional _E4_ sites, unlinked to the

translocation, which were inherited according to expectation. Esterase activities of progeny clones, measured by immunoassay, mostly corresponded to the number of amplified sites inherited,

with some discrepancies which could be attributed to copy number differences between sites, inheritance of partially methylated genes from French R, or position effect variegation at the

site on 3T. Inheritance of the A1,3 translocation in two crosses differed markedly from expectation. SIMILAR CONTENT BEING VIEWED BY OTHERS A SELF-ELIMINATING ALLELIC-DRIVE REVERSES

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Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Entomology, The Natural History Museum, London, SW7 5BD, UK R L Blackman & J M Spence *

Biological and Ecological Chemistry Department, IACR-Rothamsted, Harpenden, AL5 2JQ, Herts, UK L M Field, N Javed, G J Devine & A L Devonshire Authors * R L Blackman View author

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RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Blackman, R., Spence, J., Field, L. _et al._ Inheritance of the amplified esterase genes responsible for

insecticide resistance in _Myzus persicae_ (Homoptera: Aphididae). _Heredity_ 77, 154–167 (1996). https://doi.org/10.1038/hdy.1996.120 Download citation * Received: 05 October 1995 * Issue

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shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * esterase genes * FISH * gene

amplification * immunoassay * insecticide resistance * position effect