An analysis of coho salmon chromatin by means of c-banding, ag- and fluorochrome staining, and in situ digestion with restriction endonucleases

ABSTRACT The chromosome complement of the coho salmon (_Oncorhynchus kisutch_) has been analysed by means of C-banding, silver and fluorochrome staining, and _in situ_ digestion with

restriction endonucleases. C-banding shows heterochromatic regions in the centromeres of most chromosomes but not in the telomeric areas. The fifteenth metacentric chromosome pair contains a

large block of constitutive heterochromatin, which occupies almost all of one chromosome arm. This region is also the site where the ribosomal cistrons are located and it reacts positively

to CMA3/DA fluorochrome staining. The NORs are subject to chromosome polymorphism, which might be explicable in terms of an amplification of ribosomal cistrons. The digestion banding

patterns produced by four types of restriction endonucleases on the euchromatic and heterochromatic regions are described. Two kinds of highly repetitive DNAs can be distinguished and the

role of restriction endonucleases as a valuable tool in chromosome characterization studies, as well as in the analysis of the structure and organization of fish chromatin, are also

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Scholar  Download references ACKNOWLEDGEMENTS The authors thank Dr J. Santos, Dr C. Sentis and Dr J. Fernandez-Piqueras for their technical assistance and their valuable comments. We are

grateful to Dr L. Sanchez, Ms A. Vinas and Mr F. Nieto for their important contribution to cell culture. We also thank our colleague Dr J. Trout for his revision of the English text. This

work was funded by a CICYT Project No. PB87/0881. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Departamento de Biologia Animal, Ecología y Genética. E. Ingeniería T. Agrícola, Campus

Universitario de Almería, Almería, 04120 R Lozano * Facultad de Ciencias, Granada, 18071 C Ruiz Rejón & M Ruiz Rejón * Universidad de Granada, Spain C Ruiz Rejón & M Ruiz Rejón

Authors * R Lozano View author publications You can also search for this author inPubMed Google Scholar * C Ruiz Rejón View author publications You can also search for this author inPubMed 

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THIS ARTICLE Lozano, R., Rejón, C. & Rejón, M. An analysis of coho salmon chromatin by means of C-banding, AG- and fluorochrome staining, and _in situ_ digestion with restriction

endonucleases. _Heredity_ 66, 403–409 (1991). https://doi.org/10.1038/hdy.1991.50 Download citation * Received: 23 August 1990 * Issue Date: 01 June 1991 * DOI:

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available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * C-banding * coho salmon * fluorochrome staining * restriction

endonuclease banding