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In plants possessing a mixed selfing and random mating system, outcrossing rate t can be estimated from progeny genotype arrays. Computer simulation studies show that if there is
heterogeneity of gene frequency between subpopulations, these estimates of t are severely biased downwards, the degree of bias increasing as the true value of t increases. This effect has
been demonstrated in the field using experimental populations of Ipomoea purpurea. These field studies also provide evidence for pollen “carryover” in I. purpurea.
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