Antitumor activity of spinasterol isolated from pueraria roots

ABSTRACT We purified phytoestrogens from Pueraria root (Pueraria mirifica from Thailand and Pueraria lobata from Korea), which is used as a rejuvenating folk medicine in Thailand and China.

Dried, powdered plant material was extracted with 100% ethanol and further separated by concentration, filtration, and thin layer silica gel chromatography. Using the fractions obtained

during separation, we first investigated their cytotoxicity in several cancer cell lines from various tissues. The ethanol-extracted components (PE1, PE4) had significant antiproliferative

effects on breast cancer cell lines, including MCF-7, ZR-75-1, MDA-MB-231, SK-BR-3, and Hs578T. Second, we compared these results with the cytotoxic effects of known flavonoids, sterols, and

coumarins from Pueraria root. The known compounds were not as effective, and occurred in a different polarity region on HPLC. Third, further separation resulted in the isolation of eight

different components (Sub PE-A to -H). One of these, PE-D, affected the growth of some breast cancer cell lines (MCF-7, MDA-MB-231) in a dose- and time-dependent manner, as well as the

growth of ovarian (2774) and cervical cancer cells (HeLa). Finally, a transfection assay showed that this component had an estrogenic effect similar to 17β-estradiol, which activates both

estrogen receptor a (ER α) and ER β. The NMR analysis determined that spinasterol (stigmasta-7, 22-dien-3β-ol) is an active cytotoxic component of Pueraria root. SIMILAR CONTENT BEING VIEWED

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PLANT ENDEMIC TO WESTERN GHATS OF INDIA Article Open access 12 January 2021 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Bioscience and Biotechnology/Institute of

Bioscience, Sejong University, Seoul, Korea Gook-Che Jeon Authors * Gook-Che Jeon View author publications You can also search for this author inPubMed Google Scholar * Myoung-Soon Park View

author publications You can also search for this author inPubMed Google Scholar * Do-Young Yoon View author publications You can also search for this author inPubMed Google Scholar *

Chul-Ho Shin View author publications You can also search for this author inPubMed Google Scholar * Hong-Sig Sin View author publications You can also search for this author inPubMed Google

Scholar * Soo-Jong Um View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS This is an Open Access article distributed under the terms

of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in

any medium, provided the original work is properly cited. Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Jeon, GC., Park, MS., Yoon, DY. _et al._ Antitumor activity of

spinasterol isolated from Pueraria roots. _Exp Mol Med_ 37, 111–120 (2005). https://doi.org/10.1038/emm.2005.15 Download citation * Published: 01 April 2005 * Issue Date: 01 April 2005 *

DOI: https://doi.org/10.1038/emm.2005.15 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not

currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * breast neoplasms * estrogen replacement *

phytoestrogens * pueraria * receptors * estrogen * spinasterol * therapy