Putative markers for the detection of breast carcinoma cells in blood


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ABSTRACT The aim of this study was to investigate certain genes for their suitability as molecular markers for detection of breast carcinoma cells using the reverse transcriptase-polymerase


chain reaction (RT-PCR). RNA was prepared from MCF-7 breast carcinoma cells and peripheral blood leucocytes of healthy female volunteers. This RNA was screened for mRNA of MUC1, cytokeratin


19 (CK19) and CD44 (exons 8-11) by RT-PCR and the results validated by Southern blots. Variable degrees of expression of MUC1 and CD44 (exons 8-11) were detected in normal peripheral blood,


rendering these genes non-specific for epithelial cells and therefore unsuitable for use as markers to detect breast carcinoma cells. Although CK19 mRNA was apparently specific, it was


deemed unsuitable for use as a marker of breast cancer cells in light of its limited sensitivity. Furthermore, an attempt at using nested primers to increase sensitivity resulted in CK19


mRNA being detected after two amplification rounds in blood from healthy volunteers. Access through your institution Buy or subscribe This is a preview of subscription content, access via


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* Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS VALIDATION OF DROPLET DIGITAL PCR FOR CYTOKERATIN 19 MRNA


DETECTION IN CANINE PERIPHERAL BLOOD AND MAMMARY GLAND Article Open access 10 August 2022 LIMITATIONS AND OPPORTUNITIES OF TECHNOLOGIES FOR THE ANALYSIS OF CELL-FREE DNA IN CANCER


DIAGNOSTICS Article 31 January 2022 A MULTIPLEX BLOOD-BASED ASSAY TARGETING DNA METHYLATION IN PBMCS ENABLES EARLY DETECTION OF BREAST CANCER Article Open access 07 August 2023 AUTHOR


INFORMATION AUTHORS AND AFFILIATIONS * University Department of Surgery, Western Infirmary, Glasgow, UK EM Eltahir Authors * EM Eltahir View author publications You can also search for this


author inPubMed Google Scholar * DS Mallinson View author publications You can also search for this author inPubMed Google Scholar * GD Birnie View author publications You can also search


for this author inPubMed Google Scholar * C Hagan View author publications You can also search for this author inPubMed Google Scholar * WD George View author publications You can also


search for this author inPubMed Google Scholar * AD Purushotham View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and


permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Eltahir, E., Mallinson, D., Birnie, G. _et al._ Putative markers for the detection of breast carcinoma cells in blood. _Br J Cancer_ 77,


1203–1207 (1998). https://doi.org/10.1038/bjc.1998.203 Download citation * Issue Date: 01 April 1998 * DOI: https://doi.org/10.1038/bjc.1998.203 SHARE THIS ARTICLE Anyone you share the


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