Loss of heterozygosity occurs at the d11s29 locus on chromosome 11q23 in invasive cervical carcinoma

ABSTRACT Allelotypic detection of loss of heterozygosity (LOH) has been used to identify putative tumour-suppressor genes. Loci on human chromosome 11q23 are frequently altered in malignant

disease, and LOH has been reported at an anonymous D11S29 locus at 11q23 in a proportion of breast and ovarian cancers and malignant melanomas. Previous studies have reported a high

frequency of LOH in cervical carcinoma mapping to 11q23. Using polymerase chain reaction techniques employing probes for a recently described polymorphic dinucleotide microsatellite within

this locus, we have searched for LOH in 69 cases of invasive cervical carcinoma. Genomic material was microdissected from sections cut from archival paraffin-embedded material, using the

patients' constitutional genotype as a control Sixty-two (90%) of the cases were informative, and LOH occurred in 25/62 (40%) of tumours. Loss of an arm or single chromosome 11 is a

well-recognised event in cervical carcinoma, and by employing other microsatellite polymorphisms mapping to 11q13 and 11p11-p12 we excluded those cases with widespread allelic loss. By doing

so, LOH at D11S29 was found in 16/53 (30%) of tumours. The findings suggest a putative tumour-suppressor gene on 11q involved in cervical carcinogenesis. Access through your institution Buy

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Article 02 September 2020 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Nuffield Department of Pathology and Bacteriology, University of Oxford, John Radcliffe Hospital, UK PB Bethwaite

Authors * PB Bethwaite View author publications You can also search for this author inPubMed Google Scholar * J Koreth View author publications You can also search for this author inPubMed 

Google Scholar * CS Herrington View author publications You can also search for this author inPubMed Google Scholar * JO'D McGee View author publications You can also search for this

author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Bethwaite, P., Koreth, J., Herrington, C. _et al._ Loss of heterozygosity

occurs at the D11S29 locus on chromosome 11q23 in invasive cervical carcinoma. _Br J Cancer_ 71, 814–818 (1995). https://doi.org/10.1038/bjc.1995.157 Download citation * Issue Date: 01 April

1995 * DOI: https://doi.org/10.1038/bjc.1995.157 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is

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