A murine monoclonal antibody, moab hmsa-5, against a melanosomal component highly expressed in early stages, and common to normal and neoplastic melanocytes

ABSTRACT The melanosome is a secretory organelle unique to the melanocyte and its neoplastic counterpart, malignant melanoma. The synthesis and assembly of these intracytoplasmic organelles

is not yet fully understood. We have developed a murine monoclonal antibody (MoAb) against melanosomes isolated from human melanocytes (newborn foreskin) cultured in the presence of 12-O

tetradecanoyl phorbol-13-acetate (TPA). This MoAb, designated HMSA-5 (Human Melanosome-Specific Antigen-5) (IgG1), recognised a cytoplasmic antigen in both normal human melanocytes and

neoplastic cells, such as common and dysplastic melanocytic nevi, and malignant melanoma. None of the carcinoma or sarcoma specimens tested showed positive reactivity with MoAb HMSA-5. Under

immunoelectron microscopy, immuno-gold deposition was seen on microvesicles associated with melanosomes, and a portion of the ER-Golgi complexes. Radioimmunoprecipitation analysis showed

that the HMSA-5 reactive antigen was a glycoprotein of M(r) 69 to 73 kDa. A pulse-chase time course study showed that the amount of antigen detected by MoAb HMSA-5 decreased over a 24 h

period without significant expression on the cell surface, or corresponding appearance of the antigen in the culture supernatant. This glycoprotein appears to play a role in the early stages

of melanosomal development, and the HMSA-5 reactive epitope may be lost during subsequent maturation processes. Importantly, HMSA-5 can be identified in all forms of human melanocytes,

hence it can be considered a new common melanocytic marker even on routine paraffin sections. Access through your institution Buy or subscribe This is a preview of subscription content,

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MELANOGENESIS AND SENESCENCE Article 12 January 2022 FUSION PARTNERS OF NTRK3 AFFECT SUBCELLULAR LOCALIZATION OF THE FUSION KINASE AND CYTOMORPHOLOGY OF MELANOCYTES Article 23 September 2020

THE JOURNEY FROM MELANOCYTES TO MELANOMA Article 24 April 2023 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Division of Dermatology and Cutaneous Sciences, University of Alberta, Edmonton,

Canada JE Der Authors * JE Der View author publications You can also search for this author inPubMed Google Scholar * WT Dixon View author publications You can also search for this author

inPubMed Google Scholar * K Jimbow View author publications You can also search for this author inPubMed Google Scholar * T Horikoshi View author publications You can also search for this

author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Der, J., Dixon, W., Jimbow, K. _et al._ A murine monoclonal antibody, MoAb

HMSA-5, against a melanosomal component highly expressed in early stages, and common to normal and neoplastic melanocytes. _Br J Cancer_ 67, 47–57 (1993). https://doi.org/10.1038/bjc.1993.8

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