Green tea polyphenol epigallocatechin-3-gallate suppresses rat hepatic stellate cell invasion by inhibition of mmp-2 expression and its activation

ABSTRACT AIM: Epigallocatechin-3-gallate (EGCG) is the major component of green tea polyphenols, whose wide range of biological properties includes anti-fibrogenic activity. Matrix

metalloproteinases (MMP) that participate in extracellular matrix degradation are involved in the development of hepatic fibrosis. The present study investigates whether EGCG inhibits

activation of the major gelatinase matrix metalloproteinase-2 (MMP-2) in rat hepatic stellate cells (HSC). METHODS: The expression of MMP-2, tissue inhibitors of metalloproteinases-2

(TIMP-2), and membrane-type 1-MMP (MT1-MMP) was assessed by RT-PCR and Western blot analyses. MMP-2 activity was evaluated by zymography and MT1-MMP activity was assessed by an enzymatic

assay. HSC migration was measured by a wound healing assay and cell invasion was performed using Transwell cell culture chambers. RESULTS: The expression of MMP-2 mRNA and protein in HSC was

substantially reduced by EGCG treatment. EGCG treatment also reduced con-canavalin A (ConA)-induced activation of secreted MMP-2 and reduced MT1-MMP activity in a dose-dependent manner. In

addition, EGCG inhibited either HSC migration or invasion. CONCLUSION: The abilities of EGCG to suppress MMP-2 activation and HSC invasiveness suggest that EGCG may be useful in the

treatment and prevention of hepatic fibrosis. SIMILAR CONTENT BEING VIEWED BY OTHERS LUTEOLIN-7-DIGLUCURONIDE, A NOVEL PTP1B INHIBITOR, AMELIORATES HEPATIC STELLATE CELL ACTIVATION AND LIVER

FIBROSIS IN MICE Article 05 August 2024 PANTOPRAZOLE AMELIORATES LIVER FIBROSIS AND SUPPRESSES HEPATIC STELLATE CELL ACTIVATION IN BILE DUCT LIGATION RATS BY PROMOTING YAP DEGRADATION

Article 31 August 2021 _N_-N-BUTYL HALOPERIDOL IODIDE AMELIORATES LIVER FIBROSIS AND HEPATIC STELLATE CELL ACTIVATION IN MICE Article 23 March 2021 ARTICLE PDF REFERENCES * Friedman SL .

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INFORMATION AUTHORS AND AFFILIATIONS * Department of Hepatobiliary Surgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, China Mao-chuan Zhen, Xiao-hui Huang, 

Qian Wang, Kai Sun, Yun-jian Liu, Wen Li, Long-juan Zhang, Liang-qi Cao & Xi-ling Chen Authors * Mao-chuan Zhen View author publications You can also search for this author inPubMed 

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inPubMed Google Scholar * Kai Sun View author publications You can also search for this author inPubMed Google Scholar * Yun-jian Liu View author publications You can also search for this

author inPubMed Google Scholar * Wen Li View author publications You can also search for this author inPubMed Google Scholar * Long-juan Zhang View author publications You can also search

for this author inPubMed Google Scholar * Liang-qi Cao View author publications You can also search for this author inPubMed Google Scholar * Xi-ling Chen View author publications You can

also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Qian Wang. ADDITIONAL INFORMATION Project supported by the Science and Technology Foundation of

Guangzhou city (No 2004Z2-E0132). RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Zhen, Mc., Huang, Xh., Wang, Q. _et al._ Green tea polyphenol

epigallocatechin-3-gallate suppresses rat hepatic stellate cell invasion by inhibition of MMP-2 expression and its activation. _Acta Pharmacol Sin_ 27, 1600–1607 (2006).

https://doi.org/10.1111/j.1745-7254.2006.00439.x Download citation * Received: 24 June 2006 * Accepted: 11 July 2006 * Issue Date: 01 December 2006 * DOI:

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not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * matrix metalloproteinase * hepatic stellate cell *

green tea * migration * invasion