Efflux of potassium ion is an important reason of hl-60 cells apoptosis induced by tachyplesin

ABSTRACT AIM: To investigate the role of intercellular potassium in tachyplesin-induced HL-60 cells apoptosis. METHODS: The concentration of intercellular potassium, cell volume and

mitochondrial membrane potential were examined by flow cytometry. RESULTS: The concentration of intercellular potassium reduced in a time-dependent manner in tachyplesin-treated HL-60 cells.

In addition, the loss of mitochondrial membrane potential was tightly coupled with the shrinkage of cell volume. Different caspase inhibitors protected against DNA degradation but did not

prevent the loss of HL-60 cell viability induced by tachyplesin. Ba2+, which was a kind of blocker of volume-regulatory K+ channels, increased the viability of tachyplesin-treated HL-60

cells and maintained mitochondrial membrane potential and cell volume. CONCLUSION: Efflux of K+ was an important reason for apoptosis in tachyplesin-treated HL-60 cells. Efflux of K+

affected the viability of tachyplesin-treated HL-60 cells independent of the process of caspase activation. SIMILAR CONTENT BEING VIEWED BY OTHERS CA2+-MEDIATED MITOCHONDRIAL INNER MEMBRANE

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  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang, 524023, China

Hai-tao Zhang & Hai-feng Zhang * Affiliated Hospital of Guangdong Medical College, Zhanjiang, 524023, China Jun Wu Authors * Hai-tao Zhang View author publications You can also search

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Hai-tao Zhang. ADDITIONAL INFORMATION Project supported by the Administration of Traditional Chinese Medicine of Guangdong province (1050047). RIGHTS AND PERMISSIONS Reprints and permissions

ABOUT THIS ARTICLE CITE THIS ARTICLE Zhang, Ht., Wu, J., Zhang, Hf. _et al._ Efflux of potassium ion is an important reason of HL-60 cells apoptosis induced by tachyplesin. _Acta Pharmacol

Sin_ 27, 1367–1374 (2006). https://doi.org/10.1111/j.1745-7254.2006.00377.x Download citation * Received: 19 January 2006 * Accepted: 16 April 2006 * Issue Date: October 2006 * DOI:

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not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * horseshoe crab * tachyplesin * intracellular

potassium * mitochondrial membrane potential * HL-60 cell