Delivery of adenoviral vectors to the prostate for gene therapy

ABSTRACT Prostate cancer has become the most frequently occurring cancer and the second leading cause of cancer deaths in men. One novel approach to combat prostate cancer is gene therapy. A

replication-deficient recombinant adenoviral vector (AdRSVlacZ) expressing bacterial β-galactosidase (β-gal) (_lacZ_) under the control of the Rous sarcoma virus promoter was used to

determine which delivery route was best for the transduction of adenoviral vectors to the prostate. Using a canine model, adenoviral vectors were administered by intravenous, intra-arterial,

and intraprostatic (i.p.) injections. After injections, the expression of the _lacZ_ gene was measured in canine prostates as well as in various other organs to determine the distribution

of the disseminated adenoviral vector by (a) the percentage of cells expressing _lacZ_ _in situ_ (5-bromo-4-chloro-3-indolyl β-D-galactoside staining), (b) β-gal enzymatic activity

(colorimetric β-gal assay), and (c) polymerase chain reaction of genomic DNA using primers specific for the adenoviral genome. An i.p. injection of the adenoviral vector resulted in a

greater transduction rate and expression level of _lacZ_ in the prostate than either intravenous or intra-arterial (inferior vesical/prostatic artery) injections. Thus, an i.p. (or

intratumoral) injection seems to be the best route to treat local regional prostate cancer by viral-based gene therapy. Access through your institution Buy or subscribe This is a preview of

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REMOTE ORGANS Article Open access 12 May 2022 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Urology, Urologic Research Laboratories, University of Tennessee at Memphis,

Memphis, Tennessee Yi Lu, John Carraher, Yu Zhang, Jianling Armstrong, Jody Lerner, William P Rogers & Mitchell S Steiner Authors * Yi Lu View author publications You can also search for

this author inPubMed Google Scholar * John Carraher View author publications You can also search for this author inPubMed Google Scholar * Yu Zhang View author publications You can also

search for this author inPubMed Google Scholar * Jianling Armstrong View author publications You can also search for this author inPubMed Google Scholar * Jody Lerner View author

publications You can also search for this author inPubMed Google Scholar * William P Rogers View author publications You can also search for this author inPubMed Google Scholar * Mitchell S

Steiner View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Yi Lu. RIGHTS AND PERMISSIONS Reprints and permissions

ABOUT THIS ARTICLE CITE THIS ARTICLE Lu, Y., Carraher, J., Zhang, Y. _et al._ Delivery of adenoviral vectors to the prostate for gene therapy. _Cancer Gene Ther_ 6, 64–72 (1999).

https://doi.org/10.1038/sj.cgt.7700011 Download citation * Received: 08 December 1997 * Accepted: 04 May 1998 * Published: 22 October 1999 * Issue Date: 01 January 1999 * DOI:

https://doi.org/10.1038/sj.cgt.7700011 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not

currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * Gene therapy * prostate * adenovirus * β-galactosidase

(lacZ).