Rho-dependent cell spreading activated by e. Coli cytotoxic necrotizing factor 1 hinders apoptosis in epithelial cells

ABSTRACT Cell-cell and cell-matrix interactions play a pivotal role in numerous cell functions including cell survival and death. In this work, we report evidence that the Rho-dependent cell

spreading activated by a protein toxin from _E. COLI_, the cytotoxic necrotizing factor 1 (CNF1), is capable of hindering apoptosis in HEp-2 cells. In addition to the promotion of cell

spreading, CNF1 protects cells from the experimentally-induced rounding up and detachment and improves the ability of cells to adhere to each other and to the extracellular matrix by

modulating the expression of proteins related to cell adhesion. In particular, the expression of integrins such as α5, α6 and αv, as well as of some heterotypic and homotypic

adhesion-related proteins such as the Focal Adhesion Kinase, E-cadherin, α and β catenins were significantly increased in cells exposed to CNF1. Our results suggest, however, that the

promotion of Rho-dependent cell spreading is the key mechanism in protecting cells against apoptosis rather than cell adhesion _PER SE_. A toxin inducing cell spreading without activating

Rho, such as Cytochalasin B, was in fact ineffective in favouring cell survival. These data are of relevance (i) for the understanding of the role of the actin-dependent and especially

Rho-dependent cellular activities involved in apoptosis regulation and (ii) in providing some clues to understanding the mechanisms by which bacteria, by controlling cell fate, might exert

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INHIBIT GROWTH OF INTRACELLULAR _LISTERIA MONOCYTOGENES_ Article Open access 30 January 2025 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Ultrastructures, Istituto

Superiore di Sanità, Viale Regina Elena 299, Rome, 00161, Italy Carla Fiorentini, Paola Matarrese, Elisabetta Straface, Loredana Falzano, Gianfranco Donelli & Walter Malorni * INSERM

U452 Faculté de Médecine, Avenue de Valombrose, Nice, Cedex 2, 06107, France Patrice Boquet Authors * Carla Fiorentini View author publications You can also search for this author inPubMed 

Google Scholar * Paola Matarrese View author publications You can also search for this author inPubMed Google Scholar * Elisabetta Straface View author publications You can also search for

this author inPubMed Google Scholar * Loredana Falzano View author publications You can also search for this author inPubMed Google Scholar * Gianfranco Donelli View author publications You

can also search for this author inPubMed Google Scholar * Patrice Boquet View author publications You can also search for this author inPubMed Google Scholar * Walter Malorni View author

publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Carla Fiorentini. ADDITIONAL INFORMATION Edited by R.A. Knight RIGHTS AND

PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Fiorentini, C., Matarrese, P., Straface, E. _et al._ Rho-dependent cell spreading activated by _E.coli_ cytotoxic

necrotizing factor 1 hinders apoptosis in epithelial cells. _Cell Death Differ_ 5, 921–929 (1998). https://doi.org/10.1038/sj.cdd.4400422 Download citation * Received: 09 February 1998 *

Revised: 15 May 1998 * Accepted: 02 June 1998 * Published: 03 November 1998 * Issue Date: 01 November 1998 * DOI: https://doi.org/10.1038/sj.cdd.4400422 SHARE THIS ARTICLE Anyone you share

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Nature SharedIt content-sharing initiative KEYWORDS * apoptosis * CNF1 * actin cytoskeleton * cell adhesion * cell spreading * Rho