Peyronie's disease fibroblasts demonstrate tumorigenicity in the severe combined immunodeficient (scid) mouse model

ABSTRACT Peyronie's disease is a localized connective tissue disorder, caused by trauma to the erect penis, which results in cellular proliferation and excess extracellular matrix

production within the tunica albuginea of the penis. We have previously demonstrated that cells derived from Peyronie's disease plaque tissue demonstrate increased cell growth,

increased S-phase on flow cytometry, stabilization and inactivation of p53, and consistent morphologic transformation, all suggesting that these cells are biologically transformed. Severe

combined immunodeficient (SCID) mice have been used extensively to study the pathobiology of malignant and benign tissue and cells. This study was undertaken to determine if Peyronie's

derived fibroblasts had the potential to demonstrate tumorigenicity in the SCID mouse model, thus confirming their biologically transformed nature. Cultured fibroblasts were derived from

three sources, namely, plaque tissue excised from men with Peyronie's disease, tunical tissue excised from young men with congenital penile curvature and neonatal foreskins. BALB/C SCID

mice were divided into three groups and each group was inoculated with cultured fibroblasts from each of the three different sources. All animals were evaluated regularly and maintained in

isolation for a period of 3 months following inoculation. All SCID mice inoculated with cells derived from Peyronie's disease plaque tissue (_n_=10) developed subcutaneous nodules at a

mean time period of 2.5±0.5 months following injection. The mean maximum dimension and weight of the nodules at the time of killing the animal was 1.1±0.2 cms and 0.6±0.2 g, respectively.

Histologically, the nodules were composed of large pleomorphic epithelioid cells with a high mitotic activity, which were negative for cytokeratin but positive for vimentin. None of the SCID

mice inoculated with cells cultured from either normal tunica (_n_=5) or foreskin (_n_=5) developed subcutaneous nodules. In conclusion, the tumorigenic nature of Peyronie's disease

plaque-derived fibroblasts sheds further light on the pathobiologic characteristics of these cells. Specifically, these data confirm that cells cultured from Peyronie's disease plaque

are biologically transformed. Future refinement and study of this animal model may permit a more complete understanding of the pathophysiology of Peyronie's disease and fibromatoses in

general. Furthermore, such an animal model may, in the future, allow a more ready evaluation of the therapeutic interventions for Peyronie's disease. Access through your institution Buy

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J P Mulhall, D J Martin, T Lubrano & T V Shankey * Department of Urology, Weill Medical College, Cornell University, New York J P Mulhall, D J Martin, E Kwon & T V Shankey * The

Oncology Institute, Cardinal Bernardin Cancer Center, Maywood, Illinois, USA M Moser & E Kwon * Department of Pathology, Loyola University Medical Center, Stritch School of Medicine,

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J P Mulhall. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Mulhall, J., Martin, D., Lubrano, T. _et al._ Peyronie's disease fibroblasts

demonstrate tumorigenicity in the severe combined immunodeficient (SCID) mouse model. _Int J Impot Res_ 16, 99–104 (2004). https://doi.org/10.1038/sj.ijir.3901183 Download citation *

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KEYWORDS * Peyronie's disease * fibroblasts * fibromatosis * SCID mice * transformation