An anti-sense chalcone synthase gene in transgenic plants inhibits flower pigmentation

ABSTRACT In most plants flower pigments derive from the flavonoid biosynthesis pathway. Consistent with this pathway in _Petunia hybrida_ the key enzyme in flavonoid synthesis, chalcone

synthase, is synthesized in the flower corolla, tube and anthers1. Here we show that constitutive expression of an 'anti-sense' chalcone synthase gene in transgenic petunia and

tobacco plants results, with high frequency, in an altered flower pigmentation due to a reduction in levels of both the messenger RNA for the enzyme and the enzyme itself. The pattern of

pigmentation varies among flowers of different transgenic plants, indicating that the activity of the anti-sense gene is influenced by DNA sequences that border its site of insertion in both

a quantitative and a qualitative way. Backcrossing experiments show that the different pigmentation phenotypes resulting from the expression of anti-sense chalcone synthese gene(s) are

stably inherited. These data establish that secondary metabolism in plants can be manipulated using transgenic plants that constitutively synthesize anti-sense RNA. Access through your

institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print

issues and online access $199.00 per year only $3.90 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to

local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT

BEING VIEWED BY OTHERS SUPPRESSION OF CHORISMATE SYNTHASE, WHICH IS LOCALIZED IN CHLOROPLASTS AND PEROXISOMES, RESULTS IN ABNORMAL FLOWER DEVELOPMENT AND ANTHOCYANIN REDUCTION IN PETUNIA

Article Open access 02 July 2020 THE PLANT ALCR-_PALCA_ ETHANOL-INDUCIBLE SYSTEM DISPLAYS GROSS GROWTH ARTEFACTS INDEPENDENTLY OF DOWNSTREAM _PALCA-_REGULATED INDUCIBLE CONSTRUCTS Article

Open access 25 January 2021 CRISPR/CAS9-MEDIATED TARGETED MUTATION REVEALS A ROLE FOR _AN4_ RATHER THAN _DPL_ IN REGULATING VENATION FORMATION IN THE COROLLA TUBE OF _PETUNIA HYBRIDA_

Article Open access 01 June 2021 REFERENCES * Koes, R. E., _et al._ _Nucl. Acids Res._ 4, 5229–5239 (1986). Article  Google Scholar  * Rosenberg, U. B., Preiss, A., Seifert, E., Jäckie, H.

& Knipple, D. C. _Nature_ 313, 703–706 (1985). Article  ADS  CAS  Google Scholar  * Cabrera, C. V., Alonso, M. C., Johnston, P., Phillips, R. G. & Lawrence, P. A. _Cell_ 50, 659–663

(1987). Article  CAS  Google Scholar  * Boulay, J. L., Dennefeld, C. & Alberga, A. _Nature_ 330, 395–398 (1987). Article  ADS  CAS  Google Scholar  * Knecht, D. A. & Loomis, W. F.

_Science_ 236, 1081–1086 (1987). Article  ADS  CAS  Google Scholar  * Rothstein, S. J., DiMaio, J., Strand, M. & Rice, D. _Proc. natn. Acad. Sci. U.S.A._ 84, 8439–8443 (1987). Article 

ADS  CAS  Google Scholar  * Ecker, J. R. & Davis, R. W. _Proc. natn. Acad. Sci. U.S.A._ 83, 5372–5376 (1986). Article  ADS  CAS  Google Scholar  * Reif, H. J., Niesbach, U., Dcumling, B.

& Saedler, H. _Molec. gen. Genet._ 199, 208–215 (1985). Article  CAS  Google Scholar  * Izant, J. G. & Weintraub, H. _Science_ 229, 345–352 (1985). Article  ADS  CAS  Google Scholar

  * Bevan, M. W., Mason, S. E. & Goelet, P. _EMBO J._ 4, 1921–1926 (1985). Article  CAS  Google Scholar  * Wallroth, M., Gerats, A. G. M., Rogers, S. G., Fraley, R. T. & Horsch, R.

B. _Molec. gen. Genet._ 202, 6–15 (1986). Article  CAS  Google Scholar  * Meyer, P., Heidmann, I., Forkmann, G. & Saedler, H. _Nature_ 330, 677–678 (1987). Article  ADS  CAS  Google

Scholar  * Green, J. P., Pines, O. & Innouge, M. _A. Rev. Biochem._ 55, 569–597 (1986). Article  CAS  Google Scholar  * Jones, J. D. G., Dunsmuir, P. & Bedbrook, J. _EMBO J._ 4,

2411–2418 (1985). Article  CAS  Google Scholar  * Bevan, M. _Nucl. Acids Res._ 12, 8711–8721 (1984). Article  CAS  Google Scholar  * Ditta, G., Stanfield, S., Corbin, D. & Helinski, D.

R. _Proc. natn. Acad. Sci. U.S.A._ 77, 7347–7351 (1980) Article  ADS  CAS  Google Scholar  * Horsch, R. B. _et al_ _Science_ 227, 1229–1231 (1985). Article  ADS  CAS  Google Scholar  * Koes,

R. E., Spelt, C. E., Mol, J. N. M. & Gerats, A. G. M. _Plant molec. Biol._ 10, 159–169 (1987). Article  CAS  Google Scholar  * Mol, J. N. M. _et al._ _Molec. gen. Genet._ 192, 424–429

(1983). Article  CAS  Google Scholar  * Tunen, A. J. van & Mol, J. N. M. _Archs Biochem. Biophys._ 257, 85–91 (1987). Article  Google Scholar  Download references AUTHOR INFORMATION

AUTHORS AND AFFILIATIONS * Department of Applied Genetics, Vrije Universiteit, de Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands Alexander R. van der Krol, Peter E. Lenting, Jetty

Veenstra, Ingrid M. van der Meer, Ronald E. Koes, Anton G. M. Gerats, Joseph N. M. Mol & Antoine R. Stuitje Authors * Alexander R. van der Krol View author publications You can also

search for this author inPubMed Google Scholar * Peter E. Lenting View author publications You can also search for this author inPubMed Google Scholar * Jetty Veenstra View author

publications You can also search for this author inPubMed Google Scholar * Ingrid M. van der Meer View author publications You can also search for this author inPubMed Google Scholar *

Ronald E. Koes View author publications You can also search for this author inPubMed Google Scholar * Anton G. M. Gerats View author publications You can also search for this author inPubMed

 Google Scholar * Joseph N. M. Mol View author publications You can also search for this author inPubMed Google Scholar * Antoine R. Stuitje View author publications You can also search for

this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE van der Krol, A., Lenting, P., Veenstra, J. _et al._ An anti-sense

chalcone synthase gene in transgenic plants inhibits flower pigmentation. _Nature_ 333, 866–869 (1988). https://doi.org/10.1038/333866a0 Download citation * Received: 25 January 1988 *

Accepted: 04 May 1988 * Issue Date: 30 June 1988 * DOI: https://doi.org/10.1038/333866a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get

shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative