In vivo gene transfer to the mouse eye using an hiv-based lentiviral vector; efficient long-term transduction of corneal endothelium and retinal pigment epithelium

ABSTRACT We have evaluated the transduction profiles of an HIV-based lentiviral vector delivered regionally to ocular tissues in vivo. Following subretinal injection, a green fluorescent

protein (GFP) reporter gene was efficiently and stably expressed in retinal pigment epithelial (RPE) cells. Limited transduction of adjacent photoreceptors occurred in newborn mice, but was

inefficient in adult animals. Injection of the vector into the anterior chamber resulted in efficient and stable transduction of corneal endothelial cells. Efficient in vivo gene transfer

into cells of the corneal endothelium and retinal pigment epithelium by lentiviral vectors may therefore offer a valuable approach to the treatment of disorders of the cornea and outer

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support SIMILAR CONTENT BEING VIEWED BY OTHERS LENTIVIRAL DELIVERY OF CO-PACKAGED _CAS9_ MRNA AND A _VEGFA_-TARGETING GUIDE RNA PREVENTS WET AGE-RELATED MACULAR DEGENERATION IN MICE Article

04 January 2021 IN SITU TRANSDUCTION OF CELLS IN HUMAN CORNEAL LIMBUS USING ADENO-ASSOCIATED VIRUSES: AN EX VIVO STUDY Article Open access 28 December 2022 RAAV-PHP.B ESCAPES THE MOUSE EYE

AND CAUSES LETHALITY WHEREAS RAAV9 CAN TRANSDUCE ANIRIDIC CORNEAL LIMBAL STEM CELLS WITHOUT LETHALITY Article Open access 19 April 2023 REFERENCES * Ali RR . Gene transfer into the mouse

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JWBB is a Wellcome Trust Research Training Fellow. AJT is a Wellcome Trust Senior Clinical Fellow. KF and CD are supported by grants from the Primary Immunodeficiency Association and the

Chronic Granulomatous Disease Research Trust. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Institute of Ophthalmology, University College London, UK JWB Bainbridge, C Stephens, A Halfyard 

& RR Ali * Institute of Child Health, University College London, UK K Parsley, C Demaison, AJ Thrasher & RR Ali Authors * JWB Bainbridge View author publications You can also search

for this author inPubMed Google Scholar * C Stephens View author publications You can also search for this author inPubMed Google Scholar * K Parsley View author publications You can also

search for this author inPubMed Google Scholar * C Demaison View author publications You can also search for this author inPubMed Google Scholar * A Halfyard View author publications You can

also search for this author inPubMed Google Scholar * AJ Thrasher View author publications You can also search for this author inPubMed Google Scholar * RR Ali View author publications You

can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Bainbridge, J., Stephens, C., Parsley, K. _et

al._ _In vivo_ gene transfer to the mouse eye using an HIV-based lentiviral vector; efficient long-term transduction of corneal endothelium and retinal pigment epithelium. _Gene Ther_ 8,

1665–1668 (2001). https://doi.org/10.1038/sj.gt.3301574 Download citation * Received: 22 May 2001 * Accepted: 04 September 2001 * Published: 22 November 2001 * Issue Date: 01 November 2001 *

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currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * gene transfer * lentivirus * cornea * retina