Gene transfer to adult human lung tissue ex vivo

ABSTRACT The potential of gene therapy for treatment of lung disease remains unrealised. Early model systems often resulted in promising efficiency of gene transfer, only to prove

irreproducible in the clinic. While problems such as induction of host immune responses and duration of expression also need to be addressed, it is now widely believed that alternative,

relevant models which more accurately reflect gene transfer efficiencies in human lungs are urgently required. We report here on a human lung slice culture system to assess gene transfer to

adult lung epithelium. A lacZ-expressing adenovirus (AdCA35lacZ) was used as a reporter vector. A solution of AdCA35lacZ was instilled via bronchioles into resected lung tissue, a route

analogous to clinical administration. Following a 1 h incubation, the tissue was inflated with a 0.4% agarose solution, instilled via the same bronchioles. Once solidified, 500 μm slices of

the tissue were prepared and cultured for 4 days. β-Galactosidase staining revealed lacZ transgene expression in bronchiolar and alveolar cells of the lung slices throughout the 4 days in

culture. This system, which can also be used to study other viral and liposome vectors, could prove to be a useful alternative model for assessing gene delivery to adult human lung

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Trust and the Cancer Research Campaign. SMcB was a Marie Curie Fellow supported by the European Commission. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Pathology Department, CRC

Laboratories, University of Edinburgh Medical School, Teviot Place, Edinburgh, EH8 9AG, Scotland, UK S McBride, D Rannie & D J Harrison Authors * S McBride View author publications You

can also search for this author inPubMed Google Scholar * D Rannie View author publications You can also search for this author inPubMed Google Scholar * D J Harrison View author

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Harrison, D. Gene transfer to adult human lung tissue ex vivo. _Gene Ther_ 7, 675–678 (2000). https://doi.org/10.1038/sj.gt.3301146 Download citation * Received: 09 August 1999 * Accepted:

08 December 1999 * Published: 03 May 2000 * Issue Date: 01 April 2000 * DOI: https://doi.org/10.1038/sj.gt.3301146 SHARE THIS ARTICLE Anyone you share the following link with will be able to

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initiative KEYWORDS * gene therapy * adenovirus * human * lung