Molecular cloning and sequencing of a human hepatitis delta (δ) virus rna

ABSTRACT Human hepatitis delta (δ) virus (HDV) is a form of defective virus, which infects humans only in the presence of a co-infecting hepatitis B virus (HBV), HDV superinfection in a

chronic HBV carrier often results in severe chronic hepatitis and cirrhosis, whereas acute HDV and HBV co-infection is frequently associated with fulminant hepatitis1–3. HDV consists of a

36-nm particle; which contains an envelope with HBV surface antigen, and a nucleocapsid containing the hepatitis δ-antigen (HDAg) and an RNA genome of 1.75 kilobases (kb) (ref. 4). Recently,

the genomic RNA from an HDV serially passaged in chimpanzees has been cloned and sequenced in a study which showed that the HDV RNA is a single-stranded circular molecule with properties

similar to those of viroid or virusoid5,6. However, it is not known' whether serial passages in chimpanzees had altered the properties of human HDV. Here we report the cloning and

sequencing of an HDV RNA isolated directly from a patient with acute δ-hepatitis. The sequence showed considerable divergence (11%) from that of the chimpanzee-adapted HDV. Five open reading

frames (ORFs) of more than 100 amino acids in both genomic and anti-genomic sense were found. The largest ORF in antigenomic sense, which can code for 214 amino acids, may correspond to the

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  PubMed  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Microbiology, University of Southern California School of Medicine, Los Angeles,

California, 90033, USA Shinji Makino, Ming-Fu Chang, Chien-Kou Shieh, Toshio Kamahora, David M. Vannier & Michael M. C. Lai * Department of Pathology, University of Southern California

School of Medicine, Los Angeles, California, 90033, USA Sugantha Govindarajan Authors * Shinji Makino View author publications You can also search for this author inPubMed Google Scholar *

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Molecular cloning and sequencing of a human hepatitis delta (δ) virus RNA. _Nature_ 329, 343–346 (1987). https://doi.org/10.1038/329343a0 Download citation * Received: 18 May 1987 *

Accepted: 05 August 1987 * Issue Date: 24 September 1987 * DOI: https://doi.org/10.1038/329343a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this

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