Human antisera detect a plasmodium falciparum genomic clone encoding a nonapeptide repeat

ABSTRACT _Plasmodium falciparum_ causes malaria infections in its human host. Its wide distribution in tropical countries is a major world health problem. Before a vaccine can be produced,

the identification and characterization of parasite antigens is necessary. This can be achieved by the cloning and subsequent analysis of genes coding for parasite antigens1–4. Recently

established cDNA banks allow the expression of cDNA derived from the simian parasite _Plasmodium knowlesi_5 and _P. falciparum_6,7 in _Escherichia coli_. Recombinants encoding parasite

antigens have been identified by immunodetection in both banks. Two of them contain repetitive units of 11 (ref. 7) or 12 (ref. 5) amino acids. We describe here the construction of an

expression bank made directly from randomly generated fragments of _P. falciparum_ genomic DNA. We detect several clones which react strongly with human African immune sera. One clone

expresses an antigenic determinant composed of occasionally degenerated repeats of a peptide nonamer. Access through your institution Buy or subscribe This is a preview of subscription

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Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * ZMBH, 6900, Heidelberg, Im Neuenheimer Feld 364, FRG Michael Koenen * Institut für Genetik der Universität zu Köln,

5000, Köln, 41, FRG Artur Scherf & Benno Müller-Hill * Institut Pasteur, Paris, 75015, France Odile Mercereau, Gordon Langsley, Lise Sibilli, Philippe Dubois & Luis Pereira da Silva

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Mercereau, O. _et al._ Human antisera detect a _Plasmodium falciparum_ genomic clone encoding a nonapeptide repeat. _Nature_ 311, 382–385 (1984). https://doi.org/10.1038/311382a0 Download

citation * Received: 13 April 1984 * Accepted: 26 July 1984 * Issue Date: 27 September 1984 * DOI: https://doi.org/10.1038/311382a0 SHARE THIS ARTICLE Anyone you share the following link

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