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ABSTRACT Progress in the study of biosynthesis of cellulose (1,4-_β_-D-glucan) has been persistently hampered by the low rates of _in vitro_ synthesis obtained using cell-free preparations
derived from plant or bacterial cells capable of ample cellulose synthesis _in vivo_1. We recently reported the achievement of high rates of synthesis of 1,4-_β_-D-glucan from UDP-glucose,
using membrane preparations obtained from the bacterium _Acetobacter xylinum_. The key to the success lay in the discovery of a complex GTP-Ca2+-protein factor-mediated regulatory system for
the A. _xylinum_ UDP-glucose-_β_-D-glucosyltransferase (cellulose synthase)2,3. We now describe conditions found for an extremely efficient transfer of glucose from UDP-glucose to a
cellulosic 1,4-_β_-D-glucan product using enzyme preparations derived from a higher plant, seedlings of mung beans (_Phaseolus aureus_). The rates of enzyme activity achieved approach those
of cellulose synthesis observed _in vivo_. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access
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subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS A CELLULOSE SYNTHASE-DERIVED ENZYME CATALYSES 3-_O_-GLUCURONOSYLATION IN SAPONIN BIOSYNTHESIS
Article Open access 16 November 2020 A GTP-DRIVEN CENTRAL CARBON METABOLISM IN THE CELLULOLYTIC BACTERIUM _RUMINICLOSTRIDIUM CELLULOLYTICUM_ Article Open access 30 March 2025 CELLOBIOSE
PHOSPHORYLASE FROM _CALDICELLULOSIRUPTOR BESCII_ CATALYZES REVERSIBLE PHOSPHOROLYSIS VIA DIFFERENT KINETIC MECHANISMS Article Open access 10 March 2022 REFERENCES * Delmer, D. P. _Adv.
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INFORMATION AUTHORS AND AFFILIATIONS * Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel T. Callaghan & M.
Benziman Authors * T. Callaghan View author publications You can also search for this author inPubMed Google Scholar * M. Benziman View author publications You can also search for this
author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Callaghan, T., Benziman, M. High rates of _in vitro_ synthesis of
1,4-_β_-D-glucan in cell-free preparations from _Phaseolus aureus_. _Nature_ 311, 165–167 (1984). https://doi.org/10.1038/311165a0 Download citation * Received: 15 May 1984 * Accepted: 04
July 1984 * Issue Date: 13 September 1984 * DOI: https://doi.org/10.1038/311165a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable
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