Insulin-like growth factor ii precursor gene organization in relation to insulin gene family

ABSTRACT The insulin gene family1,2, comprised of insulin, relaxin, insulin-like growth factors I and II (IGF-I and IGF-II) and possibly the _β_-subunit of 7S nerve growth factor3,

represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in

pre-adolescent mammalian growth (see ref. 4 for review). IGF-I expression is regulated by growth hormone5,6 and mediates postnatal growth, while IGF-II appears to be induced by placental

lactogen during prenatal development6. The primary structures of both human IGFs have been determined and are closely related7,8. A polypeptide highly homologous to human IGF-II is secreted

by the rat liver cell line, BRL-3A9,10. As this polypeptide, termed multiplication stimulating activity (MSA), differs from human IGF-II by only five amino acid residues, MSA probably

represents the rat IGF-II protein11. Using molecular cloning techniques, we have isolated cDNA and chromosomal genes coding for the MSA and human IGF-II precursors, respectively. Our data,

presented here, indicate that both MSA12 and human IGF-II are synthesized initially as larger precursor molecules. The deduced preprohormones both have molecular weights (MWs) of 20,100 and

contain C-terminal propeptides of 89 amino acid residues, which we have named E-peptides. The organization of the IGF-II precursor gene is discussed in relation to that of other insulin gene

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Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS NON-GLYCOSYLATED IGF2 PROHORMONES ARE MORE MITOGENIC THAN NATIVE IGF2 Article Open access 19 August 2023 REGULATION AND

FUNCTION OF INSULIN AND INSULIN-LIKE GROWTH FACTOR RECEPTOR SIGNALLING Article 10 February 2025 STRUCTURAL BASIS FOR ASSEMBLY AND DISASSEMBLY OF THE IGF/IGFBP/ALS TERNARY COMPLEX Article

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2331–2348 (1980). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Molecular Biology, Genentech, Inc., 460 Point San Bruno

Boulevard, South San Francisco, California, 94080, USA Thomas J. Dull, Alane Gray, Joel S. Hayflick & Axel Ullrich Authors * Thomas J. Dull View author publications You can also search

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_Nature_ 310, 777–781 (1984). https://doi.org/10.1038/310777a0 Download citation * Received: 21 May 1984 * Accepted: 07 July 1984 * Issue Date: 30 August 1984 * DOI:

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