Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse

ABSTRACT An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in

explanted murine bone marrow tissue. Subsequent transplantation of the cells into lethally irradiated mice results in engraftment of the animals with donor haematopoietic tissue containing

the bacterial gene. This approach affords an efficient and rapid means of re-introducing genetically modified tissue into intact organisms and provides a system whereby the expression and

regulation of cloned genes can be followed within the context of a well characterized developmental programme. Access through your institution Buy or subscribe This is a preview of

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ELECTROTRANSFECTION PROTOCOL FOR MURINE EMBRYONIC FIBROBLASTS AND IPS CELLS Article Open access 07 August 2020 GENERATION OF MOUSE–HUMAN CHIMERIC EMBRYOS Article 02 July 2021 TRACING GENOMIC

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AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge David A. Williams, Ihor R. Lemischka 

& Richard C. Mulligan * Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, 02139, USA David A. Williams, Ihor R. Lemischka & Richard C. Mulligan * Divisions of

Hematology-Oncology, Children's Hospital, Pediatric Oncology, Dana-Farber Cancer Institute and the Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, 02115, USA

David A. Williams & David G. Nathan Authors * David A. Williams View author publications You can also search for this author inPubMed Google Scholar * Ihor R. Lemischka View author

publications You can also search for this author inPubMed Google Scholar * David G. Nathan View author publications You can also search for this author inPubMed Google Scholar * Richard C.

Mulligan View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Williams,

D., Lemischka, I., Nathan, D. _et al._ Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse. _Nature_ 310, 476–480 (1984).

https://doi.org/10.1038/310476a0 Download citation * Received: 27 January 1984 * Accepted: 01 May 1984 * Issue Date: 09 August 1984 * DOI: https://doi.org/10.1038/310476a0 SHARE THIS ARTICLE

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