Friend leukaemia virus-transformed cells, unlike normal stem cells, form spleen colonies in sl/sld mice

ABSTRACT Neoplastic cells are characterized by partial or total autonomy from the interactions that regulate the behaviour of normal cells in the intact animal. Despite the role of the host

cellular environment in governing the proliferation and differentiation of both normal and malignant cells, little is actually known about these host factors. The characterization of host

genes that influence both normal cellular processes, as well as susceptibility to tumour induction, is one approach to identifying such factors. Mice carrying two recessive mutations at the

_steel (Sl)_ locus have an environmental defect that affects both normal haematopoietic stem cell function1,2 as well as susceptibility to Friend leukaemia virus3–5. In this study, we have

used _Sl/Sl_d mice to examine whether malignant transformation by this RNA tumour virus results in a population of cells capable of proliferating even in the defective cellular

microenvironment of _Sl/Sl_d mice. We report here that late after infection, the leuk-aemic spleens of Friend virus-infected mice contain cells which, unlike normal haematopoietic stem

cells, are able to form macroscopic spleen colonies in irradiated mice of genotype _Sl/Sl_d. This observation forms the basis for the first _in vivo_ colony assay for leukaemic cells

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FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS SYNGENEIC LEUKEMIA MODELS USING LENTIVIRAL TRANSGENICS Article Open access 18 February 2021 ONCOGENE-INDUCED

SENESCENCE IN HEMATOPOIETIC PROGENITORS FEATURES MYELOID RESTRICTED HEMATOPOIESIS, CHRONIC INFLAMMATION AND HISTIOCYTOSIS Article Open access 27 July 2021 ARTIFICIAL SELECTION FOR HOST

RESISTANCE TO TUMOUR GROWTH AND SUBSEQUENT CANCER CELL ADAPTATIONS: AN EVOLUTIONARY ARMS RACE Article Open access 07 October 2020 REFERENCES * McCulloch, E. A., Siminovitch, L., Till, J. E.,

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Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * The Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto,

Toronto, Ontario, M4X 1K9, Canada Dixie Mager, Tak W. Mak & Alan Bernstein Authors * Dixie Mager View author publications You can also search for this author inPubMed Google Scholar *

Tak W. Mak View author publications You can also search for this author inPubMed Google Scholar * Alan Bernstein View author publications You can also search for this author inPubMed Google

Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Mager, D., Mak, T. & Bernstein, A. Friend leukaemia virus-transformed cells, unlike normal

stem cells, form spleen colonies in _Sl/Sl_d mice. _Nature_ 288, 592–594 (1980). https://doi.org/10.1038/288592a0 Download citation * Received: 01 August 1980 * Accepted: 16 October 1980 *

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