Freezing induced change in ligand orientation in oxycobalt-myoglobin

ABSTRACT Single crystals of oxycobalt-myoglobin were examined by electron paramagnetic resonance (EPR) spectroscopy at ambient and cryogenic temperatures. The principal values and

eigenvectors of the g-tensor and the hyperfine coupling tensor were determined. The Co—O—O bond angle was estimated to be 125° at ambient temperature. The single crystal EPR data of

oxycobalt myoglobin at 77 K showed two sets of the principal values for g and hyperfine coupling tensors and eigenvectors, indicating that the bound oxygen molecule takes two distinct

orientations. The result has demonstrated for the first time that the well defined change in the molecular orientation is induced upon freezing the biological macromolecule. Access through

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BEING VIEWED BY OTHERS WATER STABILIZES AN ALTERNATE TURN CONFORMATION IN HORSE HEART MYOGLOBIN Article Open access 13 April 2023 WATER-ORIENTED MAGNETIC ANISOTROPY TRANSITION Article Open

access 12 May 2021 IMPROVED DETECTION OF MAGNETIC INTERACTIONS IN PROTEINS BASED ON LONG-LIVED COHERENCES Article Open access 16 May 2024 REFERENCES * Yonetani, T., Yamamoto, H. &

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Google Scholar  Download references AUTHOR INFORMATION Author notes * Takashi Yonetani Present address: Abteilung Biochemie, Biozentrum der Universität Basel, Klingelbergstrasse 70, Basel,

CH-4056, Switzerland AUTHORS AND AFFILIATIONS * Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, 19104 Hiroshi Hori, 

Mássao Ikeda-Saito & Takashi Yonetani * INSERM Laboratory Unit 128, Montpellier, 34033, France Hiroshi Hori, Mássao Ikeda-Saito & Takashi Yonetani Authors * Hiroshi Hori View author

publications You can also search for this author inPubMed Google Scholar * Mássao Ikeda-Saito View author publications You can also search for this author inPubMed Google Scholar * Takashi

Yonetani View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Hori, H.,

Ikeda-Saito, M. & Yonetani, T. Freezing induced change in ligand orientation in oxycobalt-myoglobin. _Nature_ 288, 501–502 (1980). https://doi.org/10.1038/288501a0 Download citation *

Received: 20 May 1980 * Accepted: 17 September 1980 * Issue Date: 04 December 1980 * DOI: https://doi.org/10.1038/288501a0 SHARE THIS ARTICLE Anyone you share the following link with will be

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