Ca2+-activated atpase and atp-dependent calmodulin-stimulated ca2+ transport in islet cell plasma membrane

ABSTRACT Calcium is known to play an essential part in the regulation of insulin secretion in the pancreatic _β_ cell1,2. Calcium influx/efflux studies indicate that glucose promotes an

accumulation of calcium by the _β_ cell2. However, interpretation of such data is particularly difficult due to the complex compartmentalization of calcium within the cell. Although indirect

evidence using chlorotetracycline suggests that control of calcium homeostasis at the plasma membrane may be central to insulin secretion3, the mechanism by which secretagogues influence

the handling of calcium remains unknown. Despite its continuous diffusive entry, intracellular calcium is maintained in the submicromolar range4,5 by energy-dependent mechanisms5–9. One such

process which has been well characterized in erythrocytes is a plasma membrane calcium extrusion pump whose enzymatic basis is a high affinity (Ca2+ + Mg2+) ATPase9. A similar

mechanism10,11 regulated by insulin12,13 has recently been identified in adipocyte plasma membranes. We report here the presence of a high affinity (Ca2+ + Mg2+) ATPase and ATP-dependent

calmodulin-stimulated calcium transport system in rat pancreatic islet cell plasma membranes14. Access through your institution Buy or subscribe This is a preview of subscription content,

access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue Learn

more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS

OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS GLUCOSE AND NAADP TRIGGER ELEMENTARY

INTRACELLULAR Β-CELL CA2+ SIGNALS Article Open access 21 May 2021 5-IP7 IS A GPCR MESSENGER MEDIATING NEURAL CONTROL OF SYNAPTOTAGMIN-DEPENDENT INSULIN EXOCYTOSIS AND GLUCOSE HOMEOSTASIS

Article 18 October 2021 READILY RELEASABLE Β CELLS WITH TIGHT CA2+–EXOCYTOSIS COUPLING DICTATE BIPHASIC GLUCOSE-STIMULATED INSULIN SECRETION Article 26 January 2024 REFERENCES * Grodsky, G.

M. & Bennett, L. L. _Diabetes_ 15, 910–913 (1966). Article  CAS  Google Scholar  * Malaisse, W. J. _et al._ _Ann. N.Y. Acad. Sci._ 307, 562–582 (1978). Article  ADS  CAS  Google Scholar

  * Täljedal, I-B. _Biochem. J._ 178, 187–193 (1979). Article  Google Scholar  * Portzehl, H., Caldwell, P. C. & Rüegg, J. C. _Biochim. biophys. Acta_ 79, 581–591 (1966). Google Scholar

  * Baker, P. F. _Ann. N.Y. Acad. Sci._ 307, 250–268 (1978). Article  ADS  CAS  Google Scholar  * Carafoli, E. & Crompton, M. _Curr. Topics Membrane Transport_ 10, 151–216 (1978).

Article  CAS  Google Scholar  * Dipolo, R. _Nature_ 274, 390–293 (1978). Article  Google Scholar  * Dipolo, R. & Beaugé, L. _Nature_ 278, 271–273 (1979). Article  ADS  CAS  Google

Scholar  * Schatzmann, H. J. _Curr. Topics Membrane Transport_ 6, 125–168 (1974). Article  Google Scholar  * Pershadsingh, H. A. & McDonald, J. M. _J. biol. Chem._ 255, 4087–4093 (1980).

CAS  PubMed  Google Scholar  * Pershadsingh, H. A., Landt, M. & McDonald, J. M. _J. biol. Chem._ 255, 8983–8986 (1980). CAS  PubMed  Google Scholar  * Pershadsingh, H. A. &

McDonald, J. M. _Nature_ 281, 495–497 (1979). Article  ADS  CAS  Google Scholar  * Pershadsingh, H. A. & McDonald, J. M. _Diabetes_ 28, 366 (1979). Google Scholar  * McDonald, J. M.,

Pershadsingh, H. A., Bry, C. G., McDaniel, M. L. & Lacy, P. E. _Fedn Proc._ 39, 3628 (1980). Google Scholar  * Quist, E. E. & Roufogalis, B. D. _J. supramolec. Struct._ 6, 375–381

(1977). Article  CAS  Google Scholar  * Schatzmann, H. J. & Roelofsen, B. in _Biochemistry of Membrane Transport_ (eds Semenza, G. & Carafoli, E.) 121–130 (Springer, Berlin, 1977).

Google Scholar  * Black, B. L., McDonald, J. M. & Jarett, L. _Archs Biochem. Biophys._ 199, 92–102 (1980). Article  CAS  Google Scholar  * Blaustein, M. P., Ratzlaff, R. W., Kendrick, N.

C. & Schweitzer, E. S. _J. gen. Physiol._ 72, 15–41 (1978). Article  CAS  Google Scholar  * Black, B. L., Jarett, L. & McDonald, J. M. _Biochim. biophys. Acta_ 596, 359–371 (1980).

Article  CAS  Google Scholar  * Naber, S. P., Jarett, L. & Lacy, P. E. _Diabetes_ 26, 409 (1977). Google Scholar  * Duggan, P. F. _J. biol. Chem._ 252, 1620–1627 (1977). CAS  PubMed 

Google Scholar  * Formby, B., Capito, K., Egeberg, J. & Hedeskov, C. J. _Am. J. Physiol._ 230, 441–448 (1976). CAS  PubMed  Google Scholar  * Beechey, R. B., Roberten, A. M., Holloway,

C. T. & Knight, I. G. _Biochemistry_ 6, 3867–3879 (1967). Article  CAS  Google Scholar  * Sillén, L. G. & Martell, A. E. _Chem. Soc. Spec. Publ._ 25 (1971). * Atkins, G. L. _Eur. J.

Biochem._ 33, 175–180 (1973). Article  CAS  Google Scholar  * Mualem, S. & Karlish, S. J. D. _Nature_ 277, 238–240 (1979). Article  ADS  CAS  Google Scholar  * Richards, D. E., Rega, A.

F. & Garrahan, P. _J. Biochim. biophys. Acta_ 511, 194–201 (1978). Article  CAS  Google Scholar  * Duffy, M. J. & Schwarz, V. _Biochim. biophys. Acta_ 330, 294–301 (1973). Article 

CAS  Google Scholar  * Mollman, J. E. & Pleasure, D. E. _J. biol. Chem._ 255, 569–574 (1980). CAS  PubMed  Google Scholar  * Mitchell, P. _Biochem. J._ 81, 24 (1961). Google Scholar  *

Sehlin, J. _Biochem. J._ 156, 63–69 (1976). Article  CAS  Google Scholar  * Larsen, F. L. & Vincenzi, F. F. _Science_ 204, 306–308 (1979). Article  ADS  CAS  Google Scholar  * Sugden, M.

C., Christie, M. R. & Ashcroft, S. J. H. _FEBS Lett._ 105, 95–100 (1979). Article  CAS  Google Scholar  * Valverde, I., Vandermeers, A., Anjaneyulu, R. & Malaisse, W. J. _Science_

206, 225–227 (1979). Article  ADS  CAS  Google Scholar  * Sharp, G. W. G., Wiedenkeller, D. E., Kaelin, D., Siegel, E. G. & Wollheim, C. B. _Diabetes_ 29, 74–77 (1980). Article  CAS 

Google Scholar  * Wolff, D. J. & Brostrom, C. O. _Adv. Cyclic Nudeotide Res._ 11, 27–88 (1979). CAS  Google Scholar  * Lacy, P. E. & Kostianovsky, M. _Diabetes_ 16, 35–39 (1967).

Article  CAS  Google Scholar  * Seals, J. R., McDonald, J. M., Bruns, D. E. & Jarett, L. _Analyt. Biochem._ 90, 785–795 (1978). Article  CAS  Google Scholar  * Lowry, O. H., Rosebrough,

N. J., Farr, A. L. & Randall, R. J. _J. biol. Chem._ 193, 265–275 (1951). CAS  Google Scholar  * Brake, E. T., Will, P. C. & Cook, J. S. _Membrane Biochem._ 2, 17–46 (1978). Article

  CAS  Google Scholar  * Avruch, J. & Wallach, D. F. H. _Biochim. biophys. Acta_ 233, 334–337 (1971). Article  CAS  Google Scholar  * Charbonneau, H., McRorie, R. A. & Cormier, M. J.

_Fedn Proc._ 38, 232 (1979). Google Scholar  * Naber, S. P., McDonald, J. M., Jarett, L., McDaniel, M. L., Ludvigsen, C. & Lacey, P. E. _Diabetologia_ (in the press). Download

references AUTHOR INFORMATION Author notes * Harrihar A. Pershadsingh Present address: University of Missouri-Columbia, School of Medicine, Columbia, Missouri, 65212 AUTHORS AND AFFILIATIONS

* Division of Laboratory Medicine, Departments of Pathology and Medicine, Washington University School of Medicine, St Louis, Missouri, 63110 Harrihar A. Pershadsingh, Michael L. McDaniel, 

Michael Landt, Cheryl G. Bry, Paul E. Lacy & Jay M. McDonald Authors * Harrihar A. Pershadsingh View author publications You can also search for this author inPubMed Google Scholar *

Michael L. McDaniel View author publications You can also search for this author inPubMed Google Scholar * Michael Landt View author publications You can also search for this author inPubMed

 Google Scholar * Cheryl G. Bry View author publications You can also search for this author inPubMed Google Scholar * Paul E. Lacy View author publications You can also search for this

author inPubMed Google Scholar * Jay M. McDonald View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT

THIS ARTICLE CITE THIS ARTICLE Pershadsingh, H., McDaniel, M., Landt, M. _et al._ Ca2+-activated ATPase and ATP-dependent calmodulin-stimulated Ca2+ transport in islet cell plasma membrane.

_Nature_ 288, 492–495 (1980). https://doi.org/10.1038/288492a0 Download citation * Received: 28 April 1980 * Accepted: 29 September 1980 * Issue Date: 04 December 1980 * DOI:

https://doi.org/10.1038/288492a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently

available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative