Calcium requirement for axoplasmic transport in mammalian nerve

ABSTRACT TO account for fast axoplasmic transport, the movement of materials in nerve fibres, a model has been advanced in analogy to the sliding filament mechanism of muscle contraction1.

We have shown in _in vitro_ studies that transport is closely dependent on oxidative metabolism and a continual supply of ATP which could be hydrolysed by the Mg2+–Ca2+ ATPase present in

nerve2. One might, therefore, expect a dependence of transport on either Ca2+ or Mg2+. But, in previous studies of axoplasmic transport _in vitro_, we and others found transport to continue

as usual when nerves were placed in incubation media free of divalent cations3–5. Some support for the involvement of Ca2+ in transport was provided by the fact that a block of transport was

found with 50 mM oxalate, presumably by a binding of intracellular Ca2+ (ref. 1) and the block of organelle movement observed in single fibres exposed to 10 mM EDTA6. We report here that

clear evidence for a participation of Ca2+ in axoplasmic transport was revealed when a desheathed nerve preparation was used. Access through your institution Buy or subscribe This is a

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DURING DYNAMIC CONTRACTIONS IN MOUSE INTACT SKELETAL MUSCLE FIBERS Article Open access 06 January 2024 THE MOLECULAR TRANSITION THAT CONFERS VOLTAGE DEPENDENCE TO MUSCLE CONTRACTION Article

Open access 24 May 2025 ENERGY MATTERS: PRESYNAPTIC METABOLISM AND THE MAINTENANCE OF SYNAPTIC TRANSMISSION Article 15 November 2021 REFERENCES * Ochs, S. _Science_ 176, 252–260 (1972).

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references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Physiology and the Medical Biophysics Program, Indiana University School of Medicine, Indianapolis, Indiana SIDNEY

OCHS, ROBERT M. WORTH & SHEW-YIN CHAN Authors * SIDNEY OCHS View author publications You can also search for this author inPubMed Google Scholar * ROBERT M. WORTH View author

publications You can also search for this author inPubMed Google Scholar * SHEW-YIN CHAN View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND

PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE OCHS, S., WORTH, R. & CHAN, SY. Calcium requirement for axoplasmic transport in mammalian nerve. _Nature_ 270,

748–750 (1977). https://doi.org/10.1038/270748a0 Download citation * Received: 19 August 1977 * Accepted: 24 October 1977 * Published: 01 December 1977 * Issue Date: 22 December 1977 * DOI:

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