Somatic hybridisation of petunia hybrida and p. Parodii

ABSTRACT INTRASPECIFIC and interspecific fusion of plant protoplasts is induced by agents1–4. Protoplasts of several species can regenerate a cell wall and divide to form callus, from which

whole plants can be grown5. To combine these processes and obtain hybrid cells and then hybrid plants several requirements must be met6. The parental protoplasts should be capable of being

cultured, as should the hybrid cells, and plant regeneration must be possible. Selection procedures developed so far7–10 may not be generally applicable, and we have now developed a method

which makes use of naturally occurring differences in the sensitivity of cultured plant protoplasts to growth media and drugs11. Access through your institution Buy or subscribe This is a

preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access $199.00 per

year only $3.90 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during

checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS AN ASYMMETRIC

PROTOPLAST FUSION AND SCREENING METHOD FOR GENERATING CELERIAC CYBRIDS Article Open access 25 February 2021 ANALYSIS OF THE POSSIBLE CYTOGENETIC MECHANISM FOR OVERCOMING HYBRID LETHALITY IN

AN INTERSPECIFIC CROSS BETWEEN _NICOTIANA SUAVEOLENS_ AND _NICOTIANA TABACUM_ Article Open access 09 April 2021 PHENOTYPIC, MOLECULAR AND BIOCHEMICAL EVALUATION OF SOMATIC HYBRIDS BETWEEN

_SOLANUM TUBEROSUM_ AND _S. BULBOCASTANUM_ Article Open access 16 March 2022 REFERENCES * Power, J. B., Cummins, S. E., and Cocking, E. C., _Nature_, 225, 1016–1018 (1970). Article  ADS  CAS

  Google Scholar  * Keller, W. A., and Melchers, G., _Z. Naturforsch._, 28 C, 737–741 (1973). Article  CAS  Google Scholar  * Kao, K. N., and Michayluk, M. R., _Planta_, 115, 355–367 (1974).

Article  CAS  Google Scholar  * Wallin, A., Glimelius, K., and Eriksson, T., _Z. Pflanzenphysiol._, 74, 64–80 (1974). Article  CAS  Google Scholar  * Nickell, L. G., and Heinz, D. J., in

_Genes, Enzymes and Populations_ (edit. by Srb, A. M.), 109–128 (Plenum, New York, 1973). Book  Google Scholar  * Power, J. B., and Cocking, E. C., in _Applied and Fundamental Aspects of

Plant Cell, Tissue and Organ Culture_ (edit. by Reinert, J., and Bajaj, Y. P. S.), (Springer, Berlin, in the press). * Carlson, P. S., Smith, H. H., and Dearing, R. D., _Proc. natn. Acad.

Sci. U.S.A._, 69, 2292–2294 (1972). Article  ADS  CAS  Google Scholar  * Melchers, G., and Labib, G., _Molec. gen. Genet._, 135, 277–294 (1974). Article  Google Scholar  * Melchers, G.,

Keller, W., and Labib, G., in _Modification of the Information Content of Plant Cells_ (edit. by Markham, R., Davies, D. R., Hopwood, D. A., and Horne, R. W.), 161–168 (North-Holland,

Amsterdam, 1975). Google Scholar  * Gleba, Y. Y., Butenko, R. G., and Sytnik, K. M., _Dokl. Akad. Nauk. S.S.S.R._, 221, 1196–1198 (1975). Google Scholar  * Cocking, E. C., _et al._, _Pl.

Sci. Lett._, 3, 341–350 (1974). Article  CAS  Google Scholar  * Hayward, C., and Power, J. B., _Pl. Sci. Lett._, 4, 407–410 (1975). Article  Google Scholar  * Murashige, T., and Skoog, F.,

_Physiol. Pl._, 15, 473–497 (1962). Article  CAS  Google Scholar  * Frearson, E. M., Power, J. B., and Cocking, E. C., _Devl Biol._, 33, 130–137 (1973). Article  CAS  Google Scholar  *

Power, J. B., _et al._, _Pl. Sci. Lett._, 7, 51–55 (1976). Article  CAS  Google Scholar  * Sobel, J. S., Albrecht, A. M., Riehm, H., and Biedler, J. L., _Cancer Res._, 31, 297–307 (1971).

CAS  PubMed  Google Scholar  * Cocking, E. C., and Peberdy, J. F. (eds), _The Use of Protoplasts from Fungi and Higher Plants as Genetic Systems_, 67 (Department of Botany, University of

Nottingham, 1974). * Gray, J. C., and Wildman, S. G., _Pl. Sci. Lett._, 6, 91–96 (1976). Article  CAS  Google Scholar  * Chen, K., Kung, S. D., Gray, J. C., and Wildman, S. G., _Pl. Sci.

Lett._ (in the press). * Mather, K., _J. Genet._, 45, 215–235 (1943). Article  Google Scholar  * Sink, K. C., _Can. J. Genet. Cytol._, 27, 67–74 (1975). Article  Google Scholar  Download

references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * ARC Group, Department of Botany, University of Nottingham, University Park, Nottingham, NG7 2RD, UK J. B. POWER, E. M. FREARSON, C.

HAYWARD, D. GEORGE, P. K. EVANS, S. F. BERRY & E. C. COCKING Authors * J. B. POWER View author publications You can also search for this author inPubMed Google Scholar * E. M. FREARSON

View author publications You can also search for this author inPubMed Google Scholar * C. HAYWARD View author publications You can also search for this author inPubMed Google Scholar * D.

GEORGE View author publications You can also search for this author inPubMed Google Scholar * P. K. EVANS View author publications You can also search for this author inPubMed Google Scholar

* S. F. BERRY View author publications You can also search for this author inPubMed Google Scholar * E. C. COCKING View author publications You can also search for this author inPubMed 

Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE POWER, J., FREARSON, E., HAYWARD, C. _et al._ Somatic hybridisation of _Petunia hybrida_

and P. _parodii_. _Nature_ 263, 500–502 (1976). https://doi.org/10.1038/263500a0 Download citation * Received: 04 June 1976 * Accepted: 23 July 1976 * Issue Date: 07 October 1976 * DOI:

https://doi.org/10.1038/263500a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently

available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative