Loss of a critical neutral protease in ageing wi-38 cells

ABSTRACT THE limited lifespan of normal human diploid cells _in vitro_ seems to be related to the number of mitotic divisions the cells have undergone rather than to passage of time1–3. In

spite of considerable research3–7 it is still unclear what biochemical events actually mediate the cell death or more accurately the absence of growth. In other systems it has been

postulated that regulation of growth may be mediated by activity at the cell membrane. Specifically it has been suggested that cellular division is controlled in part by membrane mediated

cell contact, and proteases and lysosomal enzymes have ben implicated in the control of mitosis and growth8–11. Thus cell surface events may be either primary or secondary events mediating

cell ageing. Acid hydrolases have been postulated as modifying the cell surface in sublethal autolysis, electrophoretic mobility of cells gives an indication of cell surface chemistry and

neutral proteases have been implicated in modification of cell external surfaces9–15. We now demonstrate that two cell surface parameters are greatly affected by _in vitro_ ageing: activity

of a _p_H 7.8 protease and cell surface net charge as measured by electrophoretic mobility. It should be emphasised that the small standard deviations for the electrophoretic mobility data

reported herein are a function of the large _n_ (number of independent observations) and do not preclude population heterogeneity. Access through your institution Buy or subscribe This is a

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IN AGEING: FROM MECHANISMS TO THERAPEUTIC OPPORTUNITIES Article 16 December 2020 SCREENING OF SELECTED AGEING-RELATED PROTEINS THAT EXTEND CHRONOLOGICAL LIFE SPAN IN YEAST _SACCHAROMYCES

CEREVISIAE_ Article Open access 17 December 2021 ROLE OF SENESCENT CELLS IN THE MOTILE BEHAVIOR OF ACTIVE, NON-SENESCENT CELLS IN CONFLUENT POPULATIONS Article Open access 09 March 2022

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AUTHORS AND AFFILIATIONS * Department of Pharmacology and Toxicology, H. BRUCE BOSMANN, ROGER L. GUTHEIL JR & KENNETH R. CASE * University of Rochester Cancer Center, University of

Rochester School of Medicine and Dentistry, Rochester, New York, 14642 H. BRUCE BOSMANN, ROGER L. GUTHEIL JR & KENNETH R. CASE Authors * H. BRUCE BOSMANN View author publications You can

also search for this author inPubMed Google Scholar * ROGER L. GUTHEIL JR View author publications You can also search for this author inPubMed Google Scholar * KENNETH R. CASE View author

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CASE, K. Loss of a critical neutral protease in ageing WI-38 cells. _Nature_ 261, 499–501 (1976). https://doi.org/10.1038/261499a0 Download citation * Received: 17 November 1975 * Accepted:

01 April 1976 * Issue Date: 10 June 1976 * DOI: https://doi.org/10.1038/261499a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable

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