Inhibitor of protein synthesis isolated from ribosomes of unfertilised eggs and embryos of sea urchins

ABSTRACT IT is not yet clear what mechanisms turn on protein synthesis in the first stages of embryonic development. In the sea urchin two mechanisms are debated: activation of ribosomes1,2

and activation of mRNA3,4, possibly through adenylation5,6. An inhibitor of protein synthesis has been isolated from ribosomes of unfertilised eggs of _Paracentrotus lividus_7,8 by

extraction of ribosomes with high concentrations of salt. Metafora _et al._7 found that this inhibitor is a protein, that it is not a ribonuclease and that it decreases ribosomal binding of

a synthetic messenger (poly(U)) and of phenylalanine tRNA. They found that this inhibitor was absent from extracts of ribosomes of _P. lividus_ blastulae, suggesting that it is inactivated

or lost when the eggs are fertilised. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through

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Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS A MOLECULAR NETWORK OF CONSERVED FACTORS KEEPS RIBOSOMES DORMANT IN THE EGG Article 18 January 2023 A MALE

GERM-CELL-SPECIFIC RIBOSOME CONTROLS MALE FERTILITY Article 14 December 2022 INSIGHTS INTO THE RIBOSOME FUNCTION FROM THE STRUCTURES OF NON-ARRESTED RIBOSOME–NASCENT CHAIN COMPLEXES Article

31 October 2022 REFERENCES * Monroy, A., Maggio, R., and Rinaldi, A. M., _Proc. natn. Acad. Sci. U.S.A._, 54, 107–111 (1965). Article  ADS  CAS  Google Scholar  * Maggio, R., Vittorelli, M.

L., Caffarelli-Mormino, I., and Monroy, A., _J. molec. Biol._, 31, 621–626 (1968). Article  CAS  Google Scholar  * Stavy, L., and Gross, P. R., _Proc. natn. Acad. Sci. U.S.A._, 57, 735–742

(1967). Article  ADS  CAS  Google Scholar  * Humphreys, T., _Devl Biol._, 26, 201–208 (1971). Article  CAS  Google Scholar  * Slater, I., Gillespie, D., and Slater, D. W., _Proc. natn. Acad.

Sci. U.S.A._, 70, 406–411 (1973). Article  ADS  CAS  Google Scholar  * Wilt, F. H., _Proc. natn. Acad. Sci. U.S.A._, 70, 2345–2349 (1973). Article  ADS  CAS  Google Scholar  * Metafora, S.,

Felicetti, L., and Gambino, R., _Proc. natn. Acad. Sci. U.S.A._, 68, 600–604 (1971). Article  ADS  CAS  Google Scholar  * Gambino, R., Metafora, S., Felicetti, L., and Raisman, J.,

_Biochem. biophys. Acta_, 312, 377–391 (1973). CAS  PubMed  Google Scholar  * Giudici, G., _Developmental biology of the sea urchin embryo_, (Academic Press, New York, 1973). Google Scholar

  * Reisfeld, R. A., Lewis, U. J., and Williams, D. E., _Nature_, 195, 281–283 (1962). Article  ADS  CAS  Google Scholar  * Monroy, A., and Tyler, A., _Archs Biochem. Biophys._, 103, 431–435

(1963). Article  CAS  Google Scholar  * Infante, A. A., and Nemer, M., _Proc. natn. Acad. Sci. U.S.A._, 58, 681–688 (1967). Article  ADS  CAS  Google Scholar  * Hogan, B., and Gross, P. R.,

_J. Cell Biol._, 49, 692–701 (1971). Article  CAS  Google Scholar  * Falvey, A. K., and Staehelin, T., _J. molec. Biol._, 53, 1–19 (1970). Article  CAS  Google Scholar  * Ball, W. D.,

_Biochem. biophys. Acta._, 341, 305–317 (1974). CAS  PubMed  Google Scholar  * Markman, B., _Expl Cell Res._, 23, 197–200 (1961). Article  CAS  Google Scholar  * Berg, W. E., _Expl Cell

Res._, 49, 379–395 (1968). Article  CAS  Google Scholar  * Gross, P. R., Malken, L. I., and Moyer, W. A., _Proc. Natn. Acad. Sci. U.S.A._, 51, 407–414 (1964). Article  ADS  CAS  Google

Scholar  * Whiteley, A. H., McCarthy, B. J., and Whiteley, H. R., _Proc. natn. Acad. Sci. U.S.A._, 55, 519–525 (1966). Article  ADS  CAS  Google Scholar  * Lowry, O. H., Rosebrough, N. J.,

Farr, A. L., and Randall, R. J., _J. biol. Chem._, 193, 265–275 (1951). CAS  Google Scholar  * Whiteley, A. H., and Baltzer, F., _Publ. Staz. zool Napoli_, 30, 402–457 (1958). CAS  Google

Scholar  * Zamenhoff, S., _Methods in Enzymology_, 3, 696–704 (1957). Article  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Zoology,

University of Washington, Seattle, Washington, 98195 MERRILL B. HILLE Authors * MERRILL B. HILLE View author publications You can also search for this author inPubMed Google Scholar RIGHTS

AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE HILLE, M. Inhibitor of protein synthesis isolated from ribosomes of unfertilised eggs and embryos of sea

urchins. _Nature_ 249, 556–558 (1974). https://doi.org/10.1038/249556a0 Download citation * Received: 31 January 1974 * Revised: 13 March 1974 * Issue Date: 07 June 1974 * DOI:

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