Activation of evi1 transcripts with chromosomal translocation joining the tcrvβ locus and the evi1 gene in human acute undifferentiated leukemia cell line (kasumi-3) with a complex translocation of der(3)t(3;7;8)

ABSTRACT A cell line (Kasumi-3) established from acute myeloid leukemia (AML-M0) had unique phenotypes of undifferentiated leukemia cells with expression of both T cell and myeloid antigens.

Kasumi-3 cells with t(3;7)(q26;q22) highly expressed a 6 kb transcript of _EVI1_, which is located on chromosome 3q26. Therefore, we further characterized the chromosomal breakpoint by

pulsed-field gel electrophoresis near _EVI1_. We identified and isolated the chromosomal breakpoint at approximately 80 kb upstream from the 5′ end of _EVI1_. Sequence analysis of the

breakpoint revealed that the whole Vβ region from T cell receptor beta (TCRβ) at 7q35 was translocated to the upstream of _EVI1_. A 1.0 kb _TCR_β transcript was expressed in the Kasumi-3

cells, suggesting that _TCR_β rearrangement occurred as Dβ–Jβ joining events. Fluorescence _in situ_ hybridization analysis revealed that the inverted chromosome 7q22-q35 segment between

_TCR_β and the region proximal to the erythropoietin gene at 7q22 was translocated to the region distal to _EVI1_ in der(3). Since the telomeric region of chromosome 8 q was also

translocated to the inverted chromosome 7q22-q35 segment in der(3), the chromosomal abnormalities of der(3) were defined as being der(3)t(3;7;8)(3pter-3q26::7q35-7q22::8q22-8qter). It is

suggested that a translocated enhancer element in the _TCR_β locus and/or loss of a negative regulatory element near _EVI1_ might function to enhance the _EVI1_ expression. Therefore, the

enhanced _EVI1_ expression may contribute to the development of a subset of undifferentiated leukemia. Access through your institution Buy or subscribe This is a preview of subscription

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29 November 2022 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Biology Division, National Cancer Center Research Institute, Tokyo, Japan K Suzukawa, T Kodera, S Shimizu, J Yokota & K

Morishita * Division of Hematology, Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan K Suzukawa & T Nagasawa * Department of Cancer Cytogenetics, Research Institute

for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan H Asou & N Kamada * Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto,

Japan M Taniwaki Authors * K Suzukawa View author publications You can also search for this author inPubMed Google Scholar * T Kodera View author publications You can also search for this

author inPubMed Google Scholar * S Shimizu View author publications You can also search for this author inPubMed Google Scholar * T Nagasawa View author publications You can also search for

this author inPubMed Google Scholar * H Asou View author publications You can also search for this author inPubMed Google Scholar * N Kamada View author publications You can also search for

this author inPubMed Google Scholar * M Taniwaki View author publications You can also search for this author inPubMed Google Scholar * J Yokota View author publications You can also search

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ABOUT THIS ARTICLE CITE THIS ARTICLE Suzukawa, K., Kodera, T., Shimizu, S. _et al._ Activation of _EVI1_ transcripts with chromosomal translocation joining the _TCRVβ_ locus and the _EVI1_

gene in human acute undifferentiated leukemia cell line (Kasumi-3) with a complex translocation of der(3)t(3;7;8). _Leukemia_ 13, 1359–1366 (1999). https://doi.org/10.1038/sj.leu.2401483

Download citation * Received: 12 November 1998 * Accepted: 23 April 1999 * Published: 09 September 1999 * Issue Date: 01 September 1999 * DOI: https://doi.org/10.1038/sj.leu.2401483 SHARE

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clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * _EVI1_ * _TCRβ_ * Kasumi-3 * chromosomal breakpoint * AML (FAB-M0)