Expression of flt4 and its ligand vegf-c in acute myeloid leukemia

ABSTRACT FLT4 represents a recently cloned member of class III receptor tyrosine kinases which include receptors for the angiogenic growth factor VEGF, namely FLT1 and KDR. The ligand of

FLT4 has been identified as VEGF-C which shares sequence homology with VEGF and P1GF. In the adult FLT4 shows a restricted expression pattern that is limited to lymphatic endothelia and

endothelia of some high endothelial venules (HEV). FLT4 has also been detected in some tumor cell lines including the hematopoietic line HEL. We therefore investigated expression of FLT4 and

its ligand VEGF-C in fresh samples from patients with AML. Using a sensitive PCR method we detected FLT4 m-RNA in 15 of 41 patients with _de novo_ AML at diagnosis or relapse and in three

of 12 patients with secondary AML. FLT4 expression was confirmed by immunocytochemistry in a subgroup of the studied patient population. FLT4 was also found in leukemic cell line U937, but

not TF-1 and KG1a. VEGF-C expression was found in leukemic samples of four of seven FLT4-positive and four of six FLT4-negative patients. U937 cells also produced VEGF-C m-RNA.

Interestingly, FLT4 expression was not detected in bone marrow samples of 15 normal volunteer donors or in CD34-positive cells from three additional donors. Possible autocrine and paracrine

growth stimulation of leukemic blasts by VEGF-C is currently being investigated in our laboratory. Access through your institution Buy or subscribe This is a preview of subscription content,

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CHAIN MEDIATES ONCOGENIC ACTIVITY OF FLT3-ITD-POSITIVE AML Article Open access 08 November 2021 TARGETING _LMO2_-INDUCED AUTOCRINE FLT3 SIGNALING TO OVERCOME CHEMORESISTANCE IN EARLY T-CELL

PRECURSOR ACUTE LYMPHOBLASTIC LEUKEMIA Article Open access 23 January 2025 TLE4 IS A REPRESSOR OF THE ONCOGENIC ACTIVITY OF TLX3 IN T-CELL ACUTE LYMPHOBLASTIC LEUKEMIA Article 21 January

2025 AUTHOR INFORMATION Author notes * U Graeven Present address: Medizinische Universitätsklinik Knappschaftskrankenhaus, In der Schornau 23-25, D-44892, Bochum, Germany AUTHORS AND

AFFILIATIONS * Department of Hematology/Oncology, University Hospital Eppendorf, Hamburg, Germany W Fielder, U Graeven, M Stockschläder & DK Hossfeld * Department of Institute of

Anatomy, University Hospital Eppendorf, Hamburg, Germany S Ergün, S Verago & N Kilic Authors * W Fielder View author publications You can also search for this author inPubMed Google

Scholar * U Graeven View author publications You can also search for this author inPubMed Google Scholar * S Ergün View author publications You can also search for this author inPubMed 

Google Scholar * S Verago View author publications You can also search for this author inPubMed Google Scholar * N Kilic View author publications You can also search for this author inPubMed

 Google Scholar * M Stockschläder View author publications You can also search for this author inPubMed Google Scholar * DK Hossfeld View author publications You can also search for this

author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Fielder, W., Graeven, U., Ergün, S. _et al._ Expression of FLT4 and its

ligand VEGF-C in acute myeloid leukemia. _Leukemia_ 11, 1234–1237 (1997). https://doi.org/10.1038/sj.leu.2400722 Download citation * Received: 21 December 1996 * Accepted: 03 April 1997 *

Issue Date: 01 August 1997 * DOI: https://doi.org/10.1038/sj.leu.2400722 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link

Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * AML * VEGF-C * FLT4 *

angiogenesis