Effect of Indole-3-Acetic Acid (IAA) on Phosphorus Metabolism in the Avena Coleoptile

IT is generally believed that energy-rich phosphate compounds act as a store-house of energy for different reactions during growth. The auxin-induced stimulation of respiration is presumed

to be due to an increased availability of phosphate acceptors due to the rapid utilization of nucleotides during the growth process1–3. Rhodes and Ashworth4 have speculated on the mechanism

by which growth substances may be metabolized for the generation of energy-rich phosphate bonds, but an unequivocal demonstration of the stimulation of nucleotide synthesis is not available.

Ormrod and Williams5 and Key et al.6 have recently observed increased phosphorylating activities in plants sprayed with 2,4-dichlorophenoxyacetic acid. Marre and Forti7 had noted a rapid

increase in high-energy phosphate level in auxin-treated pea internode segments during the first 30 min., followed by a decline. The method of estimation of adenosine triphosphate (ATP)

adopted by them, which involves the adsorption of adenine nucleotides on charcoal followed by elution with hydrochloric acid, has several limitations when applied to tissue sections. Since

numerous metabolic reactions go on in the cell at the same time, and a large number of them concern the participation of nucleotides, particularly reactions mediated by the kinase type of

enzyme, and the phosphate residues are transferred to a variety of substrates, estimation of the level of adenine nucleotides at any given time is not the true measure of the total ATP

formed during the experimental period. A more correct approach is to estimate the phosphate esterified to organic compounds including the nucleotides. Phosphate labelled with phosphorus-32

may be conveniently used for this purpose and the present communication summarizes the findings of an investigation directed towards this objective.

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