Use of pectin in gel electrophoresis

ABSTRACT ELECTROPHORESIS of protein mixtures in agar gels has been described by Gordon _et al._ 1. It has been developed by Grabar and Williams and used in their immuno-electrophoretic

method2. The latter, thanks to the specificity and sensitivity of immuno-chemical reactions, permits the detection of the presence even of the smallest amounts of components in a protein

mixture and permits defining them by their electrophoretic mobilities. The agar gels can be dried and stained3, as in paper electrophoresis. However, the use of agar has one disadvantage :

the samples are usually introduced in the gel, mixed with melted agar, thus being heated to 40°–45° C. Thus there is a constant danger of denaturing certain more sensitive protein

components. A possible interaction of agar with some of the substances analysed cannot be excluded. Finally, it was of interest to find a gel from which the electrophoretically separated

components could be extracted and estimated quantitatively. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS

OPTIONS Access through your institution Subscribe to this journal Receive 51 print issues and online access $199.00 per year only $3.90 per issue Learn more Buy this article * Purchase on

SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about

institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS A SET OF SIMPLE METHODS FOR DETECTION AND EXTRACTION OF LAMINARINASE Article

Open access 28 January 2021 POMEGRANATE FRUIT JUICE ADULTERATION WITH APPLE JUICE: DETECTION BY UV–VISIBLE SPECTROSCOPY COMBINED WITH MULTIVARIATE STATISTICAL ANALYSIS Article Open access 25

March 2022 PERCHLORATE SALTS CONFER PSYCHROPHILIC CHARACTERISTICS IN Α-CHYMOTRYPSIN Article Open access 16 August 2021 REFERENCES * Gordon, A. H., Keil, B., and Šebesta, K., _Nature_, 164,

498 (1949). Gordon, A. H., Keil, B., Šebesta, K., Knessl, O., and Sorm, F., _Coll. Trav. Chim. Tchecosl._, 15, 1 (1950). Article  ADS  CAS  Google Scholar  * Grabar, P., and Williams, jun.,

C. A., _Biochim. Biophys. Acta_, 17, 67 (1955). Williams, jun., C. A., and Grabar, P., _J. Immunol._, 74, 158, 397, 404 (1955). Article  CAS  Google Scholar  * Uriel, J., and Scheidegger, J.

J., _Bull. Soc. Chim. Biol._, 37, 165 (1955). Uriel, J., and Grabar, P., _Ann. Inst. Pasteur_ [90, 427 (1956)]. CAS  PubMed  Google Scholar  * Consden, R., and Powell, M. N., _J. Clin.

Path._, 8, 150 (1955). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION Author notes * PIERRE GRABAR: Service de Chimie microbienne, Institut Pasteur, Paris; Senior

Fellow of the McLaughlin Foundation, University of Texas, Medical Branch, Galveston, Texas, for the period February–March 1955. AUTHORS AND AFFILIATIONS * Tissue Metabolism Research

Laboratory, University of Texas, Medical Branch, Galveston, Texas PIERRE GRABAR, WIKTOR W. NOWINSKI & BRUCE D. GENEREAUX Authors * PIERRE GRABAR View author publications You can also

search for this author inPubMed Google Scholar * WIKTOR W. NOWINSKI View author publications You can also search for this author inPubMed Google Scholar * BRUCE D. GENEREAUX View author

publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE GRABAR, P., NOWINSKI, W. &

GENEREAUX, B. Use of Pectin in Gel Electrophoresis. _Nature_ 178, 430 (1956). https://doi.org/10.1038/178430a0 Download citation * Issue Date: 25 August 1956 * DOI:

https://doi.org/10.1038/178430a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently

available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative