Flow cytometric assessment of lymphocyte subsets, lymphoid progenitors, and hematopoietic stem cells in allogeneic stem cell grafts

ABSTRACT Currently, bone marrow (BM), cord blood (CB), and G-CSF-mobilized peripheral blood progenitor cells (PBPCs) are the most commonly used sources for allogeneic stem cell

transplantation (SCT). The aim of this study was to assess the yields and distribution of lymphocyte subsets, lymphocyte progenitors and hematopoietic stem cells (HSC) in each type of

allograft by three-color flow cytometry. The yields of CD34+CD38− HSCs did not differ significantly between BM grafts (2.80 ± 0.74 × 106) and leukapheresis products (LPs) (1.82 ± 0.64 × 

106), and were lowest in CB grafts (0.21 ± 0.05 × 106). For most lymphocyte subsets yields were lowest in CB grafts and significantly higher in LPs than in BM grafts. BM grafts, however,

contained the highest yields of CD34+CD19+CD20− B cell progenitors and CD19+CD20− B cells. The relative frequencies of the naive CD45RA+CD45RO− phenotype among CD4+ and CD8high T cells were

highest in CB grafts (_P_ ⩽ 0.001), and higher in LPs than in BM grafts (_P_ ⩽ 0.02). The latter finding was in accordance with a preferential G-CSF mobilization of naive T cells relative to

the total lymphocyte population (_P_ ⩽ 0.014). CD3+CD8low and CD3+CD8lowCD4− subsets, which facilitate engraftment in murine transplantation models, demonstrated a tendency towards lower

frequencies among T cells in CB grafts and LPs compared to BM grafts. This observation coincided with a significantly reduced mobilization of subsets potentially enriched for facilitating

cells as compared to the total lymphocyte population (_P_ ⩽ 0.036). The CD34+ compartment of CB grafts contained a significantly higher percentage (12.1%) of CD34+CD7+CD3− T cell progenitors

than those of BM grafts (5.1%) and LPs (3.6%). In addition, CB lymphocytes contained the highest fraction of CD3−CD16/56+ NK cells (_P_ ⩽ 0.013) and almost no CD3+CD16/56+ NKT cells (_P_ 

< 0.001) compared to adult cell sources. In summary, LPs, CB allografts and BM allografts differ widely with respect to the cellular composition of their lymphocyte compartments, which is

partially affected by a varying mobilization efficiency of G-CSF for distinct lymphocyte subsets. _Bone Marrow Transplantation_ (2001) 28, 1073–1082. Access through your institution Buy or

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IMPACT OF ANTI-T-LYMPHOCYTE GLOBULIN DOSING ON GVHD AND IMMUNE RECONSTITUTION IN MATCHED UNRELATED MYELOABLATIVE PERIPHERAL BLOOD STEM CELL TRANSPLANTATION Article Open access 13 July 2022

IMPACT ON OUTCOMES OF MIXED CHIMERISM OF BONE MARROW CD34+ SORTED CELLS AFTER MATCHED OR HAPLOIDENTICAL ALLOGENEIC STEM CELL TRANSPLANTATION FOR MYELOID MALIGNANCIES Article 28 June 2022

CHARACTERISTICS OF CELLS WITH ENGRAFTMENT CAPACITY WITHIN CD34+ CELL POPULATION UPON G-CSF AND PLERIXAFOR MOBILIZATION Article 20 July 2020 REFERENCES * Gratama JW, Verdonck LF, van der

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references ACKNOWLEDGEMENTS The authors thank Niels Borregaard, Ole Soerensen, Jack Cowland and Thomas Rasmussen for their critical review of the manuscript. This work was supported in part

by the Amalie Jørgensens Memorial Foundation, by the Novo Nordisk Foundation, and by the Danish Medical Research Council. KT-M is the recipient of a scholarship from the Danish Cancer

Society and the IMK foundation. KR-J is the recipient of a scholarship from the Medical Research Council of Copenhagen (HS). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of

Hematology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark K Theilgaard-Mönch, H Palm, K Schjødt, L Vindeløv & N Jacobsen * Department of Clinical Immunology,

Rigshospitalet, University of Copenhagen, Copenhagen, Denmark K Theilgaard-Mönch, K Raaschou-Jensen & E Dickmeiss * Department of Pediatrics Clinic II, Rigshospitalet, University of

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Theilgaard-Mönch, K., Raaschou-Jensen, K., Palm, H. _et al._ Flow cytometric assessment of lymphocyte subsets, lymphoid progenitors, and hematopoietic stem cells in allogeneic stem cell

grafts. _Bone Marrow Transplant_ 28, 1073–1082 (2001). https://doi.org/10.1038/sj.bmt.1703270 Download citation * Received: 21 February 2001 * Accepted: 17 September 2001 * Published: 17

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lymphocyte subsets * facilitating cells * G-CSF mobilization * allogeneic stem cell transplantation * allografts