A Dope for Embedding Wax | Nature


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ABSTRACT ATTENTION has been directed by Higgs1 to the effect of small quantities of petroleum ceresins in causing paraffin wax to cool in a microcrystalline state. It seemed possible that


this effect might be valuable in the technique of cutting paraffin sections. During this summer we have been using, for the routine sectioning of early amphibian embryos, a paraffin wax


mixture, without addition of beeswax but containing 0.5 per cent of petroleum ceresin which was supplied by Messrs. Shell-Mex and B. P., Ltd. The results have been all that could be desired,


the wax cooling with a very fine texture even when the embedding dish was allowed to cool in the air without being immersed in water. It is necessary to use a mixture having a melting


point, before the addition of ceresin, slightly lower than is normally appropriate. The electrostatic properties of the wax appear to be unaltered. The dope may be particularly useful in


embedding large objects, in which ordinary wax tends to cool too slowly in the centre of the block. SIMILAR CONTENT BEING VIEWED BY OTHERS PARAFFIN-EMBEDDING FOR LARGE VOLUME BIO-TISSUE


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PDF REFERENCES * Higgs, P. G., _J. Inst. Petroleum Tech._, 21, 1; 1935. See also _NATURE_, 135, 113; 1935. CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND


AFFILIATIONS * Laboratory of Experimental Zoology, Cambridge C. H. WADDINGTON & J. KRIEBEL Authors * C. H. WADDINGTON View author publications You can also search for this author


inPubMed Google Scholar * J. KRIEBEL View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE


CITE THIS ARTICLE WADDINGTON, C., KRIEBEL, J. A Dope for Embedding Wax. _Nature_ 136, 685 (1935). https://doi.org/10.1038/136685b0 Download citation * Issue Date: 26 October 1935 * DOI:


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