Comparison of gene-expression profiles between diffuse- and intestinal-type gastric cancers using a genome-wide cdna microarray

ABSTRACT Gastric cancer is the fourth leading cause of cancer-related death in the world. Two histologically distinct types of gastric carcinoma, ‘intestinal’ and ‘diffuse’, have different

epidemiological and pathophysiological features that suggest different mechanisms of carcinogenesis. A number of studies have investigated intestinal-type gastric cancers at the molecular

level, but little is known about mechanisms involved in the diffuse type, which has a more invasive phenotype and poorer prognosis. To clarify the mechanisms that underlie its development

and/or progression, we compared the expression profiles of 20 laser-microbeam-microdissected diffuse-type gastric-cancer tissues with corresponding noncancerous mucosae by means of a cDNA

microarray containing 23 040 genes. We identified 153 genes that were commonly upregulated and more than 1500 that were commonly downregulated in the tumors. We also identified a number of

genes related to tumor progression. Furthermore, comparison of the expression profiles of diffuse-type with those of intestinal-type gastric cancers identified 46 genes that may represent

distinct molecular signatures of each histological type. The putative signature of diffuse-type cancer exhibited altered expression of genes related to cell–matrix interaction and

extracellular-matrix (ECM) components, whereas that of intestinal-type cancer represented enhancement of cell growth. These data provide insight into different mechanisms underlying gastric

carcinogenesis and may also serve as a starting point for identifying novel diagnostic markers and/or therapeutic targets for diffuse-type gastric cancers. Access through your institution

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OTHERS A SPATIAL TRANSCRIPTOMIC SIGNATURE OF 26 GENES RESOLVED AT SINGLE-CELL RESOLUTION CHARACTERIZES HIGH-RISK GASTRIC CANCER PRECURSORS Article Open access 25 February 2025 COMBINED

BIOINFORMATICS TECHNOLOGY TO EXPLORE PIVOT GENES AND RELATED CLINICAL PROGNOSIS IN THE DEVELOPMENT OF GASTRIC CANCER Article Open access 29 July 2021 MULTI-BIOINFORMATICS REVEALED POTENTIAL

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144–148. Download references ACKNOWLEDGEMENTS We thank Ms Tae Makino for technical assistance, Ms Emi Ichihashi for data analysis, Dr Naoki Oyaizu for reviewing the pathological data of

samples, Dr Arimichi Takabayashi, Dr Koichi Matsuo, Dr Akira Togashi, Dr Kazutaka Obama, Dr Takefumi Kikuchi, Dr Toshihiko Nishidate and Dr Soji Kakiuchi for helpful discussions and Dr Meiko

Takahashi for preparation of the manuscript. This work was supported in part by Research for the Future Program Grant (00L01402) from the Japan Society for the Promotion of Science. AUTHOR

INFORMATION AUTHORS AND AFFILIATIONS * Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan Natini Jinawath, Yoichi

Furukawa, Suguru Hasegawa, Meihua Li & Yusuke Nakamura * SNP Research Center, RIKEN (Institute of Physical and Chemical Research), Tokyo, Japan Tatsuhiko Tsunoda * Department of

Gastroenterological Surgery, Kyoto University Graduate School of Medicine, Kyoto, Japan Seiji Satoh * Department of Surgery, Cancer Institute Hospital, Tokyo, Japan Toshiharu Yamaguchi *

Department of Surgery, Sakai City Hospital, Osaka, Japan Hiroshi Imamura * Department of Surgery, Nara Hospital, Kinki University, School of Medicine, Nara, Japan Masatomo Inoue * Department

of Surgery, Sayama Hospital, Kinki University, School of Medicine, Osaka, Japan Hitoshi Shiozaki Authors * Natini Jinawath View author publications You can also search for this author

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also search for this author inPubMed Google Scholar * Seiji Satoh View author publications You can also search for this author inPubMed Google Scholar * Toshiharu Yamaguchi View author

publications You can also search for this author inPubMed Google Scholar * Hiroshi Imamura View author publications You can also search for this author inPubMed Google Scholar * Masatomo

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Scholar * Yusuke Nakamura View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Yusuke Nakamura. ADDITIONAL INFORMATION

Supplementary Information accompanies the paper on Oncogene website (http://www.nature.com/onc) SUPPLEMENTARY INFORMATION SUPPLEMENTARY MATERIAL: 1 (PDF 38 KB) SUPPLEMENTARY MATERIAL: 2 (PDF

239 KB) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Jinawath, N., Furukawa, Y., Hasegawa, S. _et al._ Comparison of gene-expression profiles between

diffuse- and intestinal-type gastric cancers using a genome-wide cDNA microarray. _Oncogene_ 23, 6830–6844 (2004). https://doi.org/10.1038/sj.onc.1207886 Download citation * Received: 27

November 2003 * Revised: 12 May 2004 * Accepted: 14 May 2004 * Published: 26 July 2004 * Issue Date: 02 September 2004 * DOI: https://doi.org/10.1038/sj.onc.1207886 SHARE THIS ARTICLE Anyone

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the Springer Nature SharedIt content-sharing initiative KEYWORDS * diffuse-type gastric cancer * cDNA microarray * laser-microbeam microdissection * expression profile