Overexpression of lrp12, a gene contained within an 8q22 amplicon identified by high-resolution array cgh analysis of oral squamous cell carcinomas

ABSTRACT Chromosome 8q amplification is a common event observed in cancer. In this study, we used high-resolution array comparative genomic hybridization to resolve two neighboring regions

on 8q that are both amplified in oral cancer. One region (at 8q24) contains the _MYC_ oncogene, which is frequently overexpressed in many cancers, while the other region (at 8q22) represents

a novel amplicon. The alignment of array comparative genomic hybridization profiles of 20 microdissected oral squamous cell carcinomas (OSCCs) revealed a ∼5 Mbp region of frequent copy

number alteration. This region harbors 16 known genes. Gene expression analysis comparing 15 microdissected OSCC with 16 normal epithelium samples revealed overexpression specific to _LRP12_

but not the neighboring genes, _dihydropyrimidinase_ and _FOG2_, suggesting that LRP12 may function as an oncogene in oral tumors. Access through your institution Buy or subscribe This is a

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Weichselbaum RR, Lippman SM and Hong WK . (1993). _N. Engl. J. Med._, 328, 184–194. Download references ACKNOWLEDGEMENTS We thank Sandra Henderson, Spencer Watson and Chad Malloff for

technical assistance, Dr Calum MacAulay for helpful discussion and Dr Marco Marra for providing BAC clones. This work was supported by scholarships from the Natural Sciences and Engineering

Research Council and Michael Smith Foundation for health research to CG and BPC, and grants from the Canadian Institute of Health Research, the National Cancer Institute of Canada and Genome

British Columbia. Tissue DNA was archived using funds from Grant R01 DE13124 from NIDCR. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * British Columbia Cancer Research Centre, Vancouver,

V5Z-1L3, BC, Canada Cathie Garnis, Bradley P Coe, Miriam P Rosin & Wan L Lam * Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, V6T-2B5, BC,

Canada Cathie Garnis, Bradley P Coe, Miriam P Rosin & Wan L Lam * Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, V6T-2B5, BC, Canada Lewei

Zhang Authors * Cathie Garnis View author publications You can also search for this author inPubMed Google Scholar * Bradley P Coe View author publications You can also search for this

author inPubMed Google Scholar * Lewei Zhang View author publications You can also search for this author inPubMed Google Scholar * Miriam P Rosin View author publications You can also

search for this author inPubMed Google Scholar * Wan L Lam View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Cathie

Garnis. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Garnis, C., Coe, B., Zhang, L. _et al._ Overexpression of LRP12, a gene contained within an 8q22

amplicon identified by high-resolution array CGH analysis of oral squamous cell carcinomas. _Oncogene_ 23, 2582–2586 (2004). https://doi.org/10.1038/sj.onc.1207367 Download citation *

Received: 24 August 2003 * Revised: 21 October 2003 * Accepted: 18 November 2003 * Published: 15 December 2003 * Issue Date: 01 April 2004 * DOI: https://doi.org/10.1038/sj.onc.1207367 SHARE

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clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * head and neck * array CGH * 8q * premalignant