Density-dependent location and interactions of truncated apc and β-catenin

ABSTRACT Adenomatous polyposis coli (APC) is a multifunctional tumour suppressor protein, central to development and the mature organism. It is mutated in most cases of colorectal cancer,

rendering it ineffective in mediating _β_-catenin degradation. We show that localization of full-length APC in colon carcinoma and noncancer cell lines is independent of cell density.

However, the location of truncated APC is a function of cell density and in high-density cells truncated APC is predominantly not nuclear. Although the distribution of truncated APC and

_β_-catenin is closely linked in subconfluent SW480 cells, at high cell density they are not colocalized. We postulated that in this cell line this could be due to an increase in _β_-catenin

bound to E-cadherin with formation of adherens junctions at high cell density. However, while in coimmunoprecipitation assays we observe an increase in binding between _β_-catenin and

E-cadherin and a corresponding decrease in binding between _β_-catenin and APC at high cell density, we did not observe a strict colocalization of _β_-catenin and E-cadherin at the membrane

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customer support SIMILAR CONTENT BEING VIEWED BY OTHERS MULTIVALENT TUMOR SUPPRESSOR ADENOMATOUS POLYPOSIS COLI PROMOTES AXIN BIOMOLECULAR CONDENSATE FORMATION AND EFFICIENT Β-CATENIN

DEGRADATION Article Open access 15 October 2020 COLORECTAL CARCINOMA PROGRESSION IS NOT INFLUENCED BY THE PSEUDOKINASE PEAK1 Article Open access 12 November 2024 TROP-2, NA+/K+ ATPASE, CD9,

PKCΑ, COFILIN ASSEMBLE A MEMBRANE SIGNALING SUPER-COMPLEX THAT DRIVES COLORECTAL CANCER GROWTH AND INVASION Article 07 February 2022 REFERENCES * Aberle H, Bauer A, Stappert J, Kispert A and

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18–27. * Zhang F, White RL and Neufeld KL . (2001). _Mol. Cell. Biol._, 21, 8143–8156. Download references ACKNOWLEDGEMENTS We thank Dr IS Näthke, University of Dundee for the kind gift of

antibody to APC (M-APC). Melanie L Davies is funded through Tenovus Cancer Research Campaign grant 35141; Gwyndaf T Roberts was supported by BBSRC grant 35086. J Wakeman was supported by a

Royal Society award and North West Cancer Research Fund. Thanks to Alysia Battersby, David Pryce and Ramsay McFarlane for critical reading of this manuscript. AUTHOR INFORMATION AUTHORS AND

AFFILIATIONS * School of Biological Sciences, University of Wales Bangor, Deiniol Road, Bangor, LL57 2UW, Gwynedd, UK Melanie L Davies, Gwyndaf T Roberts & Jane A Wakeman * School of

Biological Sciences, University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK David G Spiller Authors * Melanie L Davies View author publications You can also search for this author

inPubMed Google Scholar * Gwyndaf T Roberts View author publications You can also search for this author inPubMed Google Scholar * David G Spiller View author publications You can also

search for this author inPubMed Google Scholar * Jane A Wakeman View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to

Jane A Wakeman. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Davies, M., Roberts, G., Spiller, D. _et al._ Density-dependent location and interactions

of truncated APC and _β_-catenin. _Oncogene_ 23, 1412–1419 (2004). https://doi.org/10.1038/sj.onc.1207266 Download citation * Received: 16 July 2003 * Revised: 22 September 2003 * Accepted:

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content-sharing initiative KEYWORDS * adenomatous polyposis coli * _β_-catenin * cell density * E-cadherin * tumour progression