Distinct rat proteins can recognize ccaat-homologous sequences of the metallothionein promoter and trans-activate this promoter

ABSTRACT We have previously purified and characterized a rat liver protein C′BP-1 that is either identical or closely related to C/EBPδ (). The mouse metallothionein-I (MT-I) promoter

contains two C′BP-1 binding sites, one of which includes the MRE-c′ region (−135 to −110). The C′BP-1 binding activity was detected by EMSA as a major activity for MRE-c′ in nonproliferating

adult liver cells but not in rat hepatoma cells. ln this study, we purified and characterized a factor, C′BP-2, which had a dominant binding activity for MRE-c′ in Morris hepatoma 3924A, a

poorly differentiated, fast-growing tissue. C′BP-2 is a 28 kDa protein which exists in solution as a monomer. As observed for C′BP-1, affinity-purified C′BP-2 stimulated transcription from

the mMT-I gene promoter. DNase I footprinting revealed two C′BP-2 binding sites in the regions that overlap with the CCAAT homologies of the C′BP-1 binding sites on the mMT-I promoter.

C′BP-2 made essential contacts with the CCAAT homology and in the region upstream of this sequence. Competition electrophoretic mobility shift assay and methylation interference analysis

revealed that C′BP-2 is a protein closely related, but not identical, to CP2. These data suggest that C′BP-1 and C′BP-2 may play a role in hepatocyte proliferation and/or differentiation.

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TANDEM ARRAYS TO BIND CLUSTERED SHORT SEQUENCE MOTIFS Article Open access 17 April 2025 AUTHOR INFORMATION Author notes * Lyudmila P Aniskovitch Present address: Department of Biochemistry,

Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey, USA AUTHORS AND AFFILIATIONS * Department of

Medical Biochemistry, The Ohio State University College of Medicine, Columbus, 43210, Ohio, USA Samson T Jacob * Department of Pharmacology and Molecular Biology, The Chicago Medical

School, 3333 Green Bay Road, North Chicago, 60064, Illinois, USA Lyudmila P Aniskovitch Authors * Lyudmila P Aniskovitch View author publications You can also search for this author inPubMed

 Google Scholar * Samson T Jacob View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE

THIS ARTICLE Aniskovitch, L., Jacob, S. Distinct rat proteins can recognize CCAAT-homologous sequences of the metallothionein promoter and trans-activate this promoter. _Oncogene_ 16,

1475–1486 (1998). https://doi.org/10.1038/sj.onc.1201659 Download citation * Received: 06 August 1997 * Revised: 17 October 1997 * Accepted: 20 October 1997 * Published: 31 March 1998 *

Issue Date: 19 March 1998 * DOI: https://doi.org/10.1038/sj.onc.1201659 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link

Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * metallothionein-I

promoter activation * hepatoma protein * liver protein