E2f-3 accumulation is regulated by polypeptide stability

ABSTRACT E2F is a complex family of transcription factors which appears to regulate the transcription of genes required for the S phase of the mammalian cell cycle. In the present work, we

have examined the mechanisms regulating E2F-3 accumulation in mouse fibroblasts. We have determined that E2F-3 DNA binding activity is restricted to the G1/S transition and S phase in both

normal BALB/c-3T3 fibroblasts and in an SV40 virus-transformed BALB/c-3T3 derivative. Immunoblot analysis indicates that G0 and G1 cells have little or no E2F-3 polypeptide and that the

increase in the DNA binding activity of E2F-3 at the G1/S boundary is reflected by an increase in total E2F-3 protein. In contrast to the E2F-3 polypeptide, RNAse protection assays

demonstrate that the E2F-3 mRNA is clearly present in G0 and G1 cells. Finally, pulse/chase experiments indicate that the half-life of E2F-3 is approximately 40-fold greater in cells blocked

in S phase relative to asynchronously growing cells. Together, these results indicate that the accumulation E2F-3 at S phase may be regulated, at least in part, at the level of protein

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support SIMILAR CONTENT BEING VIEWED BY OTHERS E2F-DEPENDENT TRANSCRIPTION DETERMINES REPLICATION CAPACITY AND S PHASE LENGTH Article Open access 14 July 2020 HCF-1 PROMOTES CELL CYCLE

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Open access 14 December 2023 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biochemistry and Molecular Biology, Molecular Oncology Program, H Lee Moffitt Cancer Center and

Research Institute, University of South Florida, College of Medicine, Tampa, 33612, Florida, USA Aurea M Flores, Robert F Kassatly & W Douglas Cress Authors * Aurea M Flores View author

publications You can also search for this author inPubMed Google Scholar * Robert F Kassatly View author publications You can also search for this author inPubMed Google Scholar * W Douglas

Cress View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Flores, A.,

Kassatly, R. & Cress, W. E2F-3 accumulation is regulated by polypeptide stability. _Oncogene_ 16, 1289–1298 (1998). https://doi.org/10.1038/sj.onc.1201633 Download citation * Received:

05 August 1997 * Revised: 10 October 1997 * Accepted: 10 October 1997 * Published: 19 March 1998 * Issue Date: 12 March 1998 * DOI: https://doi.org/10.1038/sj.onc.1201633 SHARE THIS ARTICLE

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by the Springer Nature SharedIt content-sharing initiative KEYWORDS * cell cycle * E2F * protein stability * Rb