Identification of a brca1-associated kinase with potential biological relevance

ABSTRACT A biochemical approach was used to identify proteins which interact with human BRCA1. Through this work, a kinase activity which co-purifies with BRCA1 has been identified. This

kinase activity, which phosphorylates BRCA1 _IN VITRO_, was originally identified in Sf9 insect cells but is also present in cells of human origin including breast and ovarian carcinoma cell

lines. The BRCA1 kinase activity _IN VITRO_ is associated with a fragment of BRCA1 encompassing amino acids 329–435. This peptide is also phosphorylated in various human cell lines. A

computer-assisted sequence analysis revealed that this peptide was a potential substrate for phosphorylation by PKA, PKC, or CKII. However, phosphorylation by these kinases could not be

demonstrated _IN VITRO_ indicating the presence of another kinase activity. Phosphorylation _IN VITRO_ requires a minimal domain of BRCA1 encompassing amino acids 379–408. Notably, deletion

of this minimal domain abolishes growth suppression by BRCA1 indicating that this domain, as well as phosphorylation within this domain, may be important for BRCA1 function. Access through

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Article 22 October 2021 REAL TIME CHARACTERIZATION OF THE MAPK PATHWAY USING NATIVE MASS SPECTROMETRY Article Open access 16 April 2025 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Cancer

Research, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, 46285, Indiana, USA Teresa F Burke, Kimberley S Cocke, Stephanie J Lemke & Richard P

Beckmann * Biotechnology Divisions, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, 46285, Indiana, USA Ed Angleton & Gerald W Becker Authors *

Teresa F Burke View author publications You can also search for this author inPubMed Google Scholar * Kimberley S Cocke View author publications You can also search for this author inPubMed 

Google Scholar * Stephanie J Lemke View author publications You can also search for this author inPubMed Google Scholar * Ed Angleton View author publications You can also search for this

author inPubMed Google Scholar * Gerald W Becker View author publications You can also search for this author inPubMed Google Scholar * Richard P Beckmann View author publications You can

also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Burke, T., Cocke, K., Lemke, S. _et al._

Identification of a BRCA1-associated kinase with potential biological relevance. _Oncogene_ 16, 1031–1040 (1998). https://doi.org/10.1038/sj.onc.1201623 Download citation * Received: 14 May

1997 * Revised: 03 October 1997 * Accepted: 06 October 1997 * Published: 05 March 1998 * Issue Date: 26 February 1998 * DOI: https://doi.org/10.1038/sj.onc.1201623 SHARE THIS ARTICLE Anyone

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Springer Nature SharedIt content-sharing initiative KEYWORDS * breast cancer * BRCA1 * phosphorylation