Contrasting grain-Zn response of biofortification rice (Oryza sativa L.) breeding lines to foliar Zn application - GOV.UK

Enriching grain Zn concentration in rice through Zn fertilization is one approach to Zn biofortification efforts. Experiments to understand the effectiveness of foliar Zn application at

different growth stages in enriching grain Zn of biofortification breeding lines were conducted during the dry season at an experiment station and in a farmer's field with moderately and

severely Zn-deficient soils, respectively. Foliar Zn applications at 4 kg Zn ha−1 as zinc sulfate were given at different growth stages: active tillering, heading, and both active tillering

+ heading. Foliar Zn application at active tillering had no effect on brown rice Zn concentration, but, at heading, there was a range in genotype response from 1 to 10 mg kg−1 increase, with

an average increase of 4 mg kg−1 at the moderately Zn-deficient site and 7 mg kg−1 at the severely Zn-deficient site. At the moderately Zn-deficient site, two biofortification breeding

lines (IR83668, IR91152AC) reached the target of 30 mg kg−1 Zn in brown rice without Zn fertilization at heading stage, while two other genotypes (IR68144, IR91143AC) reached the target with

heading-stage foliar Zn application. At the severely Zn-deficient site, only one of the tested genotypes (IR68144) reached the target Zn concentration even after foliar Zn application at

both active tillering and heading stage and the same genotype recorded the greatest response to foliar Zn (10 mg kg−1 increase). Greater total leaf area at spraying time increased the

effectiveness of foliar Zn application at the severely Zn-deficient site only. Foliar Zn application at the two tested growth stages failed to overcome agronomic Zn deficiency. Three of the

biofortification breeding lines (IR68144, IR85800, and IR83668) had high grain Zn content that was independent of grain yield. In a separate experiment to test a wider range of spraying

times at the severely deficient site with IR64, the spraying at the early milk stage emerged as the most effective stage for increasing brown rice Zn concentration. Our results show that

agronomic Zn biofortification through foliar Zn application is likely to be much more effective at increasing grain Zn concentration of genotypes with strong Zn-remobilization capacity than

those with weak remobilization capacity.

Mabesa, R.L.; Impa, S.M.; Grewal, D.; Johnson-Beebout, S.E. Contrasting grain-Zn response of biofortification rice (Oryza sativa L.) breeding lines to foliar Zn application. Field Crops

Research (2013) 149: 223-233. [DOI: 10.1016/j.fcr.2013.05.012]

Contrasting grain-Zn response of biofortification rice (Oryza sativa L.) breeding lines to foliar Zn application