Gene tech helps dandelions ooze rubber › news in science (abc science)

News in Science Thursday, 17 September 2009 Eric Bland Discovery News ------------------------- A hectare of genetically modified dandelions could produce 500 to 1000 kilograms of latex

_(Source: iStockphoto)_ Researchers have developed a genetically engineered dandelion that produces more latex that could be used in gloves, tyres and drugs. If you pop the head off a

dandelion, white sap oozes freely from the wound for about a second. But if you pop the head off the genetically engineered variety, the sap oozes for minutes, producing five times more

latex than from the average dandelion. "We have identified the enzyme responsible for the rapid polymerisation and have switched it off," says Dr Dirk Prufer, a scientist at

Fraunhofer Institute in Munich, Germany, who is developing the technology. "If the plants were to be cultivated on a large scale, every hectare would produce 500 to 1000 kilograms of

latex per growing season." For thousands of years, most of the world's rubber has come from tropical rubber trees. A diagonal cut in the trunk allows the white latex to drip into

hanging cans, which can then be harvested and eventually turned into a variety of materials. But natural rubber contains trace amounts of biological impurities. For car tyre makers, those

impurities give vulcanised rubber a give and elasticity they can't get anywhere else. For some hospital workers, however, those same impurities can trigger life-threatening allergic

reactions. Synthetic or petroleum-based rubber typically has fewer impurities than natural rubber which makes it ideal for applications like allergy-free gloves. ELIMINATING POLYMERISATION

Dandelion-derived latex has both the elasticity of natural rubber but lacks the allergens, making it an ideal alternative to rubber tree latex. Unfortunately, dandelion-derived latex is also

difficult to obtain. Because dandelion latex transforms from a liquid to a solid on contact with the air (known as polymerisation), turpentine and naphtha are usually required to chemically

extract the latex from the shredded remains of Russian dandelions. To eliminate polyphenol oxidase, the enzyme responsible for the phase change, the German scientists engineered a special

virus. Once inside, the virus deleted the offending genetic sequence from the Russian dandelion's DNA. Pop the head off an infected dandelion, and the latex begins to flow freely.

Eliminating polymerisation means dandelion latex can be harvested using a low-speed centrifuge, a much easier and cheaper alternative than chemical solvents. It also means up to five times

the amount of rubber can be harvested than with chemical extraction. Allergy-free latex is only one potential use for dandelion-derived latex. If the gene that eliminates polymerisation can

be knocked out, genes that produce other enzymes can be added using the same method. Among the enzymes that researchers could add include insulin for diabetics, artificial sugars and

pharmaceutical drugs for other diseases. GM RESISTANCE But adding genes is more controversial than removing genes. Using viral vectors ensures that virtually any attempt to plant harvestable

amounts of transgenic dandelions will come up against fierce resistance from groups opposed to genetically modified crops. Which is why the German researchers are attempting to try and

breed the offending dandelion gene using traditional breeding methods. "We did it for a gene in the potato," says Prufer. "It took us about five years though." William

Ravlin, a scientist at Ohio State University who also works on dandelion-derived rubber, thinks five years is too long. Genetic modification of plants is a pariah in Europe, but Americans

tend to have a more liberal attitude towards GM, especially when it comes to plants that don't produce food. The elimination of polyphenol oxidase "sounds like a very good

discovery," says Ravlin. "Genetic modification of dandelions will probably speed up the number of potential applications, but it's not completely necessary either."

Ravlin's own research has focused on finding strains of dandelions that produce larger amounts of rubber in latex, a work he describes as "incremental." If economically

feasible, the work of both Ravlin and Prufer could quickly become exponential. "If, say, one dandelion has 100 seeds (and they usually have many more than that), you now have 100

plants, which are now producing 100 seeds each," says Ravlin. "You can see how rapidly that could ramp up production. But first you have to get to that first plant, and that will

take a lot of effort." TAGS: plastics-and-rubber, biology, botany, gm-technology Email the editor Use these social-bookmarking links to share GENE TECH HELPS DANDELIONS OOZE RUBBER.